Monroe J E, Calender A, Mulder C
Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655.
J Virol. 1988 Sep;62(9):3497-500. doi: 10.1128/JVI.62.9.3497-3500.1988.
Human immunodeficiency virus type 1 (HIV-1) can infect CD4+ lymphocytes, monocytes-macrophages, and various other cell lines, including B-cell lines. To study the parameters of B-cell infections, we examined the susceptibility of 24 B-lymphoid cell lines to both HIV-1 and HIV-2 infections. These cell lines included a series of Epstein-Barr virus (EBV) genome-negative Burkitt's lymphoma cell lines and their EBV-converted counterparts. To infect these cells we used two HIV-1 isolates and one HIV-2 isolate. Infections were monitored with a cytoplasmic RNA dot-blot and a syncytium assay. HIV infection was also studied by a novel method based on electrophoresis of DNA liberated from cells that were lysed in situ in the well of an agarose gel. All human B-cell lines could be infected with HIV-1, regardless of the presence of EBV genomes; thus, EBV infection had no major effect on HIV susceptibility of B-cell lines. Integrated proviral HIV genomes could be detected by Southern blot analysis of DNA extracted from long-term, non-HIV-producing B-cell lines. This study suggests that B-lymphoid cells may serve as reservoirs for latent or persistent HIV infections in vivo, even in the absence of EBV infection.
1型人类免疫缺陷病毒（HIV-1）可感染CD4+淋巴细胞、单核细胞-巨噬细胞以及包括B细胞系在内的各种其他细胞系。为了研究B细胞感染的参数，我们检测了24种B淋巴细胞系对HIV-1和HIV-2感染的易感性。这些细胞系包括一系列爱泼斯坦-巴尔病毒（EBV）基因组阴性的伯基特淋巴瘤细胞系及其EBV转化的对应细胞系。为了感染这些细胞，我们使用了两种HIV-1分离株和一种HIV-2分离株。通过细胞质RNA斑点印迹法和多核细胞检测法监测感染情况。还通过一种基于对在琼脂糖凝胶孔中原位裂解的细胞释放的DNA进行电泳的新方法研究了HIV感染。所有人类B细胞系均可被HIV-1感染，无论是否存在EBV基因组；因此，EBV感染对B细胞系的HIV易感性没有重大影响。通过对从长期不产生HIV的B细胞系中提取的DNA进行Southern印迹分析，可以检测到整合的前病毒HIV基因组。这项研究表明，即使在没有EBV感染的情况下，B淋巴细胞也可能作为体内潜伏或持续HIV感染的储存库。
