Choo Sung-Sik, Kang Ee-Seul, Song Inbeom, Lee Donghyun, Choi Jeong-Woo, Kim Tae-Hyung
School of Integrative Engineering, Chung-Ang University, Seoul 06974, Korea.
Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 04109, Korea.
Sensors (Basel). 2017 Apr 14;17(4):861. doi: 10.3390/s17040861.
The detection of dopamine in a highly sensitive and selective manner is crucial for the early diagnosis of a number of neurological diseases/disorders. Here, a report on a new platform for the electrochemical detection of dopamine with a considerable accuracy that comprises a 3D porous graphene oxide (pGO)/gold nanoparticle (GNP)/pGO composite-modified indium tin oxide (ITO) is presented. The pGO was first synthesized and purified by ultrasonication and centrifugation, and it was then further functionalized on the surface of a GNP-immobilized ITO electrode. Remarkably, owing to the synergistic effects of the pGO and GNPs, the 3D pGO-GNP-pGO-modified ITO electrode showed a superior dopamine-detection performance compared with the other pGO- or GNP-modified ITO electrodes. The linear range of the newly developed sensing platform is from 0.1 μM to 30 μM with a limit of detection (LOD) of 1.28 μM, which is more precise than the other previously reported GO-functionalized electrodes. Moreover, the 3D pGO-GNP-pGO-modified ITO electrodes maintained their detection capability even in the presence of several interfering molecules (e.g., ascorbic acid, glucose). The proposed platform of the 3D pGO-GNP-pGO-modified ITO electrode could therefore serve as a competent candidate for the development of a dopamine-sensing platform that is potentially applicable for the early diagnosis of various neurological diseases/disorders.
以高灵敏度和高选择性检测多巴胺对于多种神经疾病/障碍的早期诊断至关重要。在此，本文报道了一种用于电化学检测多巴胺的新平台，该平台具有相当高的准确度，它由三维多孔氧化石墨烯（pGO）/金纳米颗粒（GNP）/pGO复合材料修饰的氧化铟锡（ITO）组成。首先通过超声处理和离心合成并纯化pGO，然后将其在固定有GNP的ITO电极表面进一步功能化。值得注意的是，由于pGO和GNP的协同作用，与其他pGO或GNP修饰的ITO电极相比，三维pGO-GNP-pGO修饰的ITO电极表现出卓越的多巴胺检测性能。新开发的传感平台的线性范围为0.1 μM至30 μM，检测限（LOD）为1.28 μM，这比其他先前报道的GO功能化电极更精确。此外，即使存在几种干扰分子（例如抗坏血酸、葡萄糖），三维pGO-GNP-pGO修饰的ITO电极仍保持其检测能力。因此，所提出的三维pGO-GNP-pGO修饰的ITO电极平台可作为开发多巴胺传感平台的有力候选者，该平台有可能应用于各种神经疾病/障碍的早期诊断。
