Lightner D A, Wooldridge T A, McDonagh A F
Proc Natl Acad Sci U S A. 1979 Jan;76(1):29-32. doi: 10.1073/pnas.76.1.29.
Absorption of light converts bilirubin-IXalpha in solution to a mixture of what are probably cis-trans geometric isomers. This reaction is much faster than other photochemical reactions of bilirubin and reaches photoequilibrium before losses due to photooxidation are significant. At room temperature in the dark in the presence of trifluoroacetic acid or iodine or simply on standing, the photoproducts revert to the natural isomer. They also revert under visible light. Their formation and reversion can be followed by chromatography on polyamide and by absorbance difference spectroscopy.
光的吸收将溶液中的胆红素 - IXα 转化为可能是顺反几何异构体的混合物。该反应比胆红素的其他光化学反应快得多，并且在光氧化导致的损失显著之前达到光平衡。在室温下黑暗中，在三氟乙酸或碘存在下，或者仅仅放置时，光产物会恢复为天然异构体。它们在可见光下也会恢复。它们的形成和恢复可以通过聚酰胺色谱法和吸光度差光谱法进行跟踪。
