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两种来自子实体的低分子量多糖的纯化、理化性质及抗氧化活性

Purification, Physicochemical Properties, and Antioxidant Activities of Two Low-Molecular-Weight Polysaccharides from Fruiting Bodies.

作者信息

Gao Xiong, Qi Jiayi, Ho Chi-Tang, Li Bin, Xie Yizhen, Chen Shaodan, Hu Huiping, Chen Zhongzheng, Wu Qingping

机构信息

State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.

Guangdong Yuewei Edible Fungi Technology Co. Ltd., Guangzhou 510663, China.

出版信息

Antioxidants (Basel). 2021 Jul 20;10(7):1145. doi: 10.3390/antiox10071145.

DOI:10.3390/antiox10071145
PMID:34356378
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8301108/
Abstract

Two low-molecular-weight polysaccharides (GLP-1 and GLP-2) were purified from fruiting bodies, and their physicochemical properties and antioxidant activities were investigated and compared in this study. The results showed that GLP-1 and GLP-2 were mainly composed of mannose, glucose, galactose, xylose, and arabinose, with weight-average molecular weights of 6.31 and 14.07 kDa, respectively. Additionally, GLP-1 and GLP-2 had a similar chain conformation, crystal structure, and molecular surface morphology. Moreover, GLP-1 exhibited stronger antioxidant activities than GLP-2 in five different assays: 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), hydroxyl radical, superoxide anion radical, ferric reducing antioxidant power (FRAP), and oxygen radical antioxidant capacity (ORAC). The main linkage types of GLP-1 were found to be →4)--D-Glc-(1→, →4)--D-Glc-(1→, →3)--D-Glc-(1→, →6)--D-Gal-(1→, →6)--D-Glc-(1→, →4,6)--D-Glc-(1→, and Glc-(1→ by methylation analysis and nuclear magnetic resonance (NMR) spectroscopy. In addition, GLP-1 could protect NIH3T3 cells against tert-butyl hydroperoxide (tBHP)-induced oxidative damage by increasing catalase (CAT) and glutathione peroxidase (GSH-Px) activities, elevating the glutathione/oxidized glutathione (GSH/GSSG) ratio, and decreasing the malondialdehyde (MDA) level. These findings indicated that GLP-1 could be explored as a potential antioxidant agent for application in functional foods.

摘要

从子实体中纯化出两种低分子量多糖（GLP-1和GLP-2），并在本研究中对其理化性质和抗氧化活性进行了研究和比较。结果表明，GLP-1和GLP-2主要由甘露糖、葡萄糖、半乳糖、木糖和阿拉伯糖组成，重均分子量分别为6.31 kDa和14.07 kDa。此外，GLP-1和GLP-2具有相似的链构象、晶体结构和分子表面形态。而且，在2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)（ABTS）、羟基自由基、超氧阴离子自由基、铁还原抗氧化能力（FRAP）和氧自由基抗氧化能力（ORAC）这五种不同的测定中，GLP-1表现出比GLP-2更强的抗氧化活性。通过甲基化分析和核磁共振（NMR）光谱发现，GLP-1的主要连接类型为→4)--D-葡萄糖-(1→、→4)--D-葡萄糖-(1→、→3)--D-葡萄糖-(1→、→6)--D-半乳糖-(1→、→6)--D-葡萄糖-(1→、→4,6)--D-葡萄糖-(1→和葡萄糖-(1→。此外，GLP-1可通过提高过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的活性、提高谷胱甘肽/氧化型谷胱甘肽（GSH/GSSG）比值以及降低丙二醛（MDA）水平来保护NIH3T3细胞免受叔丁基过氧化氢（tBHP）诱导的氧化损伤。这些发现表明，GLP-1可作为一种潜在的抗氧化剂用于功能性食品。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a07/8301108/f87067cedd04/antioxidants-10-01145-g012.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a07/8301108/f87067cedd04/antioxidants-10-01145-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a07/8301108/5546cc476e24/antioxidants-10-01145-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a07/8301108/12c3408b2356/antioxidants-10-01145-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a07/8301108/edf1bc30d7f7/antioxidants-10-01145-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a07/8301108/23d2d149d922/antioxidants-10-01145-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a07/8301108/2cdd378a2599/antioxidants-10-01145-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a07/8301108/e10f8ab0de3d/antioxidants-10-01145-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a07/8301108/b2b45fd9af43/antioxidants-10-01145-g009.jpg
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