完整人肺泡巨噬细胞中血管紧张素转换酶活性的测定及吸烟的影响。

Measurement of angiotensin-converting enzyme activity in intact human alveolar macrophages and effect of smoking.

作者信息

Sugiyama Y, Yotsumoto H, Okabe T, Takaku F

机构信息

3rd Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.

出版信息

Respiration. 1988;53(3):153-7. doi: 10.1159/000195408.

DOI:10.1159/000195408

PMID:2843977

Abstract

Serum angiotensin-converting enzyme (ACE) activity is known to be elevated in various granulomatous conditions such as sarcoidosis, whose characteristic epithelioid cells are thought to belong to the macrophage series. It was recently shown that alveolar macrophages had ACE activity in their sonicated and homogenate forms. We investigated ACE activity of human alveolar macrophages in the intact form using a sensitive radioimmunoassay of generated angiotensin II. We also investigated the effect of smoking on ACE activity of alveolar macrophages using this method. Alveolar macrophages generated angiotensin II (218 +/- 106 pg/20 min/10(5) cells). More angiotensin II was generated in smokers (246 +/- 119 pg/20 min/10(5) cells) than in nonsmokers (160 +/- 50 pg/20 min/10(5) cells) (p less than 0.05). These data showed that ACE activity of macrophages is higher in smokers than nonsmokers.

摘要

已知血清血管紧张素转换酶（ACE）活性在多种肉芽肿性疾病中会升高，如结节病，其特征性上皮样细胞被认为属于巨噬细胞系列。最近有研究表明，肺泡巨噬细胞以超声破碎和匀浆形式存在时具有ACE活性。我们使用一种灵敏的放射免疫分析法检测生成的血管紧张素II，研究了完整形式的人肺泡巨噬细胞的ACE活性。我们还使用该方法研究了吸烟对肺泡巨噬细胞ACE活性的影响。肺泡巨噬细胞生成血管紧张素II（218±106 pg/20分钟/10⁵个细胞）。吸烟者（246±119 pg/20分钟/10⁵个细胞）生成的血管紧张素II比不吸烟者（160±50 pg/20分钟/10⁵个细胞）更多（p<0.05）。这些数据表明，吸烟者巨噬细胞的ACE活性高于不吸烟者。