Benning T L, McCormack K M, Ingram P, Kaplan D L, Shelburne J D
Department of Pathology, Duke University Medical Center, Durham, NC 27710.
Arch Dermatol. 1988 Oct;124(10):1541-4.
We describe the histochemical, ultrastructural, and microanalytical features of a skin biopsy specimen obtained from a patient with chlorpromazine pigmentation. Golden-brown pigment granules were present in the dermis, predominantly in a perivascular arrangement. The granules stained positively with the Fontana-Masson stain for silver-reducing substances and negatively with Perl's stain for iron. Electron microscopy revealed dense inclusion bodies in dermal histiocytes, pericytes, endothelial cells, and Schwann cells, as well as lying free in the extracellular matrix. These "chlorpromazine bodies" were quite dense even in unosmicated, unstained ultrathin sections, indicating that the pigmentation is related, at least in part, to the inclusions. Microprobe analysis of the chlorpromazine bodies revealed a striking peak for sulfur, which strongly suggests the presence of the drug or its metabolite within these inclusions.
我们描述了从一名患有氯丙嗪色素沉着患者身上获取的皮肤活检标本的组织化学、超微结构和微量分析特征。真皮中存在金棕色色素颗粒，主要呈血管周围排列。这些颗粒用用于检测还原性物质的Fontana-Masson染色呈阳性，而用用于检测铁的Perl染色呈阴性。电子显微镜显示真皮组织细胞、周细胞、内皮细胞和施万细胞中有致密的包涵体，也有游离于细胞外基质中的包涵体。即使在未用锇酸固定、未染色的超薄切片中，这些“氯丙嗪小体”也相当致密，表明色素沉着至少部分与这些包涵体有关。对氯丙嗪小体的微探针分析显示硫有一个明显的峰值，这强烈表明这些包涵体内存在该药物或其代谢产物。
