来自小皮伞属BBKAV79菌株对海军蓝HER（活性蓝171）染料的脱色及生物降解作用
Decolourization and biodegradation of Navy blue HER (Reactive Blue 171) dye from Marasmius sp. BBKAV79.
作者信息
Vantamuri Adiveppa B, Kaliwal Basappa B
机构信息
Post Graduate Department of Studies and Research in Biotechnology and Microbiology, Karnatak University, Dharwad, 580003, India.
Post Graduate Department of Studies and Research in Biotechnology and Microbiology, Davangere University, Davangere, 577 002, India.
出版信息
3 Biotech. 2017 May;7(1):48. doi: 10.1007/s13205-017-0673-1. Epub 2017 Apr 25.
White rot fungus Marasmius sp. BBKAV79 (GenBank accession number-KP455496, KP455497) exhibited decolourization and degradation of Navy blue HER dye (concentration 50 mg l) within 24 h under shaking condition. In the present study, various investigated parameters like initial dye concentration, pH, temperature, carbon and nitrogen sources were optimized to develop a faster decolourization process by Marasmius sp. BBKAV79. High-performance liquid chromatography, Fourier transform infrared spectroscopy and gas chromatography and mass spectroscopy analysis of the extracted product confirmed the biodegradation of Navy blue HER. The microbial toxicity and phytotoxicity assay revealed that the degradation of Navy blue HER produced nontoxic metabolites.
白腐真菌Marasmius sp. BBKAV79（GenBank登录号-KP455496、KP455497）在振荡条件下24小时内可使海军蓝HER染料（浓度50毫克/升）脱色并降解。在本研究中，对初始染料浓度、pH值、温度、碳源和氮源等各种研究参数进行了优化，以开发Marasmius sp. BBKAV79更快的脱色过程。对提取产物进行的高效液相色谱、傅里叶变换红外光谱以及气相色谱和质谱分析证实了海军蓝HER的生物降解。微生物毒性和植物毒性测定表明，海军蓝HER的降解产生了无毒代谢物。
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