2-脱氧-2-[¹⁸F]氟-D-葡萄糖的机器人合成：一种使用四丁基铵[¹⁸F]氟化物的常规合成方法。

Robotic production of 2-deoxy-2-[18F]fluoro-D-glucose: a routine method of synthesis using tetrabutylammonium [18F]fluoride.

作者信息

Brodack J W, Dence C S, Kilbourn M R, Welch M J

机构信息

Edward Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, MO 63110.

出版信息

Int J Rad Appl Instrum A. 1988;39(7):699-703. doi: 10.1016/0883-2889(88)90060-3.

DOI:10.1016/0883-2889(88)90060-3

PMID:2844702

Abstract

Using existing robotic hardware and software programs developed for the synthesis of several positron-emitting radiopharmaceuticals for PET imaging [Brodack et al. (1988) Appl. Radiat. Isot. 39, 689], the additional automated synthesis of 2-deoxy-2-[18F]fluoro-D-glucose (2-[18F]FDG) has been incorporated into our Zymate Laboratory Automation System. The robotic synthesis of 2-[18F]FDG took less than one week to implement, including the organization of software subroutines and construction of an additional heating station. The end of synthesis yield (12-17%) and radiochemical purity (96-99%) for the robotic preparation of 2-[18F]FDG is similar to that of the manual synthesis. This automated method uses anhydrous tetrabutylammonium [18F]fluoride as the reactive fluoride source in the labeling step. The procedure is a modification of the synthesis reported by Hamacher et al. [Hamacher et al. (1986) J. Nucl. Med. 27, 235].

摘要

利用现有的为合成多种用于正电子发射断层显像（PET）成像的放射性药物而开发的机器人硬件和软件程序[布罗达克等人（1988年），《应用辐射与同位素》，第39卷，第689页]，2-脱氧-2-[¹⁸F]氟-D-葡萄糖（2-[¹⁸F]FDG）的额外自动化合成已被纳入我们的Zymate实验室自动化系统。2-[¹⁸F]FDG的机器人合成实施耗时不到一周，包括软件子程序的编排和一个额外加热站的构建。机器人制备2-[¹⁸F]FDG的合成终产物产率（12 - 17%）和放射化学纯度（96 - 99%）与手动合成相似。这种自动化方法在标记步骤中使用无水四丁基铵[¹⁸F]氟化物作为活性氟源。该程序是对哈马彻等人[哈马彻等人（1986年），《核医学杂志》，第27卷，第235页]报道的合成方法的改进。