Jensen Victoria N, Romer Shannon H, Turner Sarah M, Crone Steven A
Neuroscience Graduate Program, University of Cincinnati.
Department of Biological Sciences, Wright State University.
J Vis Exp. 2017 Apr 17(122):55599. doi: 10.3791/55599.
Accessory respiratory muscles help to maintain ventilation when diaphragm function is impaired. The following protocol describes a method for repeated measurements over weeks or months of accessory respiratory muscle activity while simultaneously measuring ventilation in a non-anesthetized, freely behaving mouse. The technique includes the surgical implantation of a radio transmitter and the insertion of electrode leads into the scalene and trapezius muscles to measure the electromyogram activity of these inspiratory muscles. Ventilation is measured by whole-body plethysmography, and animal movement is assessed by video and is synchronized with electromyogram activity. Measurements of muscle activity and ventilation in a mouse model of amyotrophic lateral sclerosis are presented to show how this tool can be used to investigate how respiratory muscle activity changes over time and to assess the impact of muscle activity on ventilation. The described methods can easily be adapted to measure the activity of other muscles or to assess accessory respiratory muscle activity in additional mouse models of disease or injury.
当膈肌功能受损时，辅助呼吸肌有助于维持通气。以下方案描述了一种在数周或数月内重复测量辅助呼吸肌活动的方法，同时在未麻醉、自由活动的小鼠中测量通气。该技术包括手术植入无线电发射器，并将电极导线插入斜角肌和斜方肌以测量这些吸气肌的肌电图活动。通过全身体积描记法测量通气，并通过视频评估动物运动，并使其与肌电图活动同步。本文展示了在肌萎缩侧索硬化小鼠模型中进行肌肉活动和通气测量的结果，以说明该工具如何用于研究呼吸肌活动随时间的变化，并评估肌肉活动对通气的影响。所描述的方法可以很容易地进行调整，以测量其他肌肉的活动，或评估其他疾病或损伤小鼠模型中的辅助呼吸肌活动。
