海藻糖对纯外周血干细胞冷冻保存的影响。
Effect of trehalose on cryopreservation of pure peripheral blood stem cells.
作者信息
Martinetti Daniela, Colarossi Cristina, Buccheri Simona, Denti Gabriella, Memeo Lorenzo, Vicari Luisa
机构信息
Cell Biology Unit, IOM Ricerca Srl, Viagrande, I-95029 Catania, Italy.
Department of Experimental Oncology, Mediterranean Institute of Oncology, Viagrande, I-95029 Catania, Italy.
出版信息
Biomed Rep. 2017 Mar;6(3):314-318. doi: 10.3892/br.2017.859. Epub 2017 Feb 14.
Abstract
Stem cells are an important tool for the study of hematopoiesis. Despite developments in cryopreservation, post-thaw cell death remains a considerable problem. Cryopreservation protocol should limit cell damage due to freezing and ensure the recovery of the functional cell characteristics after thawing. Thus, the use of cryoprotectants is essential. In particular, the efficacy of trehalose has been reported for clinical purposes in blood stem cells. The aim of the current study was to establish an efficient method for biological research based on the use of trehalose, to cryopreserve pure peripheral blood stem cells. The efficacy of trehalose was assessed and the cell viability was evaluated. The data indicate that trehalose improves cell survival after thawing compared with the standard freezing procedure. These findings could suggest the potential for future trehalose application for research purposes in cell cryopreservation.
摘要
干细胞是造血研究的重要工具。尽管在冷冻保存方面取得了进展，但解冻后细胞死亡仍然是一个相当严重的问题。冷冻保存方案应限制冷冻导致的细胞损伤，并确保解冻后功能细胞特性的恢复。因此，使用冷冻保护剂至关重要。特别是，海藻糖在血液干细胞临床应用中的功效已有报道。本研究的目的是基于海藻糖的使用建立一种用于生物学研究的有效方法，以冷冻保存纯外周血干细胞。评估了海藻糖的功效并评价了细胞活力。数据表明，与标准冷冻程序相比，海藻糖可提高解冻后的细胞存活率。这些发现可能提示未来海藻糖在细胞冷冻保存研究目的中的应用潜力。
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