Singh Sushil Kumar, Khajuria Robinka, Kaur Loveleen
School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, 1440111, India.
3 Biotech. 2017 May;7(1):69. doi: 10.1007/s13205-017-0684-y. Epub 2017 Apr 27.
Unrestricted and reckless use of antibiotics has resulted in their accumulation in environment. This, in turn, has led to the emergence of multiple drug-resistant microbes. The present study focuses on degradation of ciprofloxacin (CIP) by an edible white rot fungus Pleurotus ostreatus. Effect of CIP was determined on radial growth and biomass of P. ostreatus. Titrimetric and spectrophotometric assays were carried out to assess the degrading potential of P. ostreatus towards CIP. It was found that CIP has a stimulatory effect on growth and enzyme activity of P. ostreatus. Maximum enzyme (glucanase, ligninases, laccase) production was observed at the highest concentration of CIP (500 ppm). Antibiotic degradation of about 68.8, 94.25 and 91.34% was estimated after 14 days of incubation at 500 ppm CIP using Titrimetric, Indigo carmine and Methyl orange assay, respectively. Degradation of CIP was further validated by high performance liquid chromatography (HPLC) and microbiological analysis. HPLC analysis revealed 95.07% degradation while microbiological test also exhibited a decreased antimicrobial activity of degraded products against Escherichia coli, Staphylococcus aureus and Streptococcus pyogenes. To the best of our knowledge, this is the first study wherein P. ostreatus was used for the degradation of ciprofloxacin.
抗生素的无节制和鲁莽使用导致其在环境中积累。这反过来又导致了多重耐药微生物的出现。本研究聚焦于可食用白腐真菌糙皮侧耳对环丙沙星（CIP）的降解。测定了CIP对糙皮侧耳径向生长和生物量的影响。进行了滴定法和分光光度法测定，以评估糙皮侧耳对CIP的降解潜力。结果发现，CIP对糙皮侧耳的生长和酶活性具有刺激作用。在CIP最高浓度（500 ppm）下观察到最大酶（葡聚糖酶、木质素酶、漆酶）产量。使用滴定法、靛蓝胭脂红法和甲基橙法分别在500 ppm CIP下孵育14天后，估计抗生素降解率分别约为68.8%、94.25%和91.34%。通过高效液相色谱（HPLC）和微生物分析进一步验证了CIP的降解情况。HPLC分析显示降解率为95.07%，而微生物测试也表明降解产物对大肠杆菌、金黄色葡萄球菌和化脓性链球菌的抗菌活性降低。据我们所知，这是首次使用糙皮侧耳降解环丙沙星的研究。
