Nates S, Ferreira Centeno A, Márquez A, Zapata M
Instituto de Virología Dr. José M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
Rev Argent Microbiol. 1987 Oct-Dec;19(4):153-9.
The assay of specific immunoglobulin M (IgM) antibodies could aid in the diagnosis of early viral infection. Here we described the results obtained for IgG and IgM separation on a small column of Sephadex A-50 ion exchanger and the subsequent measure of hemagglutination inhibition antibodies for rubella virus. This diagnostic procedure combines the efficiency, rapidity and simplicity of a chromatography method with the detection of specific antibodies by reference method. The average immunoglobulin recuperation was 91% for the immunoglobulin G (IgG) and 70% for the IgM. Eighteen sera obtained from patients with presumptive diagnostic compatible with primary rubella infection, 10 from patients with known immune status for rubella virus, and 3 sera with specific IgM for BK virus, were comparatively studied by gel filtration. Sephadex G-200, enzyme immunoassay for rubella IgM antibody and Sephadex A-50 ion exchanger. The results indicated that the proposed method can be satisfactorily used for the diagnostic of primary rubella virus infection.
特异性免疫球蛋白M（IgM）抗体检测有助于早期病毒感染的诊断。在此，我们描述了在一小柱Sephadex A - 50离子交换剂上进行IgG和IgM分离以及随后检测风疹病毒血凝抑制抗体的结果。该诊断程序将色谱法的高效、快速和简便性与参考方法检测特异性抗体相结合。免疫球蛋白G（IgG）的平均回收率为91%，IgM为70%。通过凝胶过滤对18份来自疑似原发性风疹感染患者的血清、10份已知风疹病毒免疫状态患者的血清以及3份针对BK病毒具有特异性IgM的血清进行了比较研究。Sephadex G - 200、风疹IgM抗体酶免疫测定法和Sephadex A - 50离子交换剂。结果表明，所提出的方法可令人满意地用于原发性风疹病毒感染的诊断。
