通过葡聚糖凝胶G 200凝胶过滤检测风疹特异性IgM：二硫苏糖醇的应用及氯化锰-肝素预处理的局限性（作者译）
[Detection of rubella specific IgM on gel filtration through Sephadex G 200: use of dithiothreitol and limits with MnCl2-heparine pretreatment (author's transl)].
作者信息
Freymuth F, Daon F, Vergnaud M, Valdazo A
出版信息
Ann Biol Clin (Paris). 1979;37(2):79-82.
The detection of rubella haemagglutination inhibiting antibody, in the IgM fraction of the serum, on gel filtration through Sephadex G 200, needs precautions to exclude false results. Treatment with dithiothreitol is a satisfactory method for confirming the content of rubella IgM antibody. The failure of MnCl2-heparin pretreatment to remove non specific inhibitors of rubella hemagglutinin is unfrequent (7/108) and so do be repeated. Rarely (1/108) aggregated IgG fractionates with IgM and yield false positive results.
在血清IgM组分中，通过Sephadex G 200凝胶过滤检测风疹血凝抑制抗体时，需要采取预防措施以排除假结果。用二硫苏糖醇处理是确认风疹IgM抗体含量的一种令人满意的方法。氯化锰 - 肝素预处理未能去除风疹血凝素的非特异性抑制剂的情况很少见（108例中有7例），因此无需重复进行。极少数情况下（108例中有1例），聚集的IgG与IgM一起分离并产生假阳性结果。
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