Kumar Pradeep, Srivastava Pooja, Gupta Ankit, Bajpai Manish
Department of Physiology, KGMU, Lucknow, Uttar Pradesh, India.
Department of Biotechnology, AKTU, Lucknow, Uttar Pradesh, India.
Indian J Pharmacol. 2017 Jan-Feb;49(1):116-118. doi: 10.4103/0253-7613.201031.
Electrocardiogram (ECG) is an important tool for the study of cardiac electrophysiology both in human beings and experimental animals. Existing methods of ECG recording in small animals like rat have several limitations and ECG recordings of the anesthetized rat lack validity for heart rate (HR) variability analysis. The aim of the present study was to validate the ECG data from new device with ECG of anesthetized rat.
The ECG was recorded on student's physiograph (BioDevice, Ambala) and suitable coupler and electrodes in six animals first by the newly developed device in conscious state and second in anesthetized state (stabilized technique).
The data obtained were analyzed using unpaired -test showed no significant difference ( < 0.05) in QTc, QRS, and HR recorded by new device and established device in rats.
No previous study describes a similar ECG recording in conscious state of rats. Thus, the present method may be a most physiological and inexpensive alternative to other methods. In this study, the animals were not restrained; they were just secured and represent a potential strength of the study.
心电图（ECG）是研究人类和实验动物心脏电生理学的重要工具。现有的大鼠等小动物心电图记录方法存在若干局限性，且麻醉大鼠的心电图记录缺乏用于心率（HR）变异性分析的有效性。本研究的目的是验证新设备记录的心电图数据与麻醉大鼠的心电图数据的一致性。
首先使用新开发的设备在清醒状态下，然后在麻醉状态下（稳定技术），在六只动物身上通过学生生理记录仪（BioDevice，安巴拉）以及合适的耦合器和电极记录心电图。
使用非配对检验对获得的数据进行分析，结果显示新设备和已有的设备在大鼠身上记录的QTc、QRS和HR无显著差异（<0.05）。
之前没有研究描述过在大鼠清醒状态下进行类似的心电图记录。因此，本方法可能是其他方法中最符合生理情况且成本低廉的替代方法。在本研究中，动物没有受到束缚；它们只是被固定住，这代表了本研究的一个潜在优势。
