Loureiro-Dias M C
Laboratory of Microbiology, Gulbenkian Institute of Science, Oeiras, Portugal.
Antonie Van Leeuwenhoek. 1988;54(4):331-43. doi: 10.1007/BF00393524.
In 248 strains representing 205 yeast species, changes in pH coupled to glucose addition were followed in unbuffered cell suspensions. Alkalinization of the external medium elicited by glucose, indicating a H+-glucose symport was observed in 34% of the strains, most of them belonging to the genera Rhodotorula, Hansenula and Candida. H+ uptake coupled to glucose transport was observed only after exhaustion of glucose in growth media. This observation was taken as an indication that, in general, the synthesis of H+-glucose symport is under the control of catabolite repression. Subsequently to the addition of glucose, in most yeasts (82%) acidification was observed. This ability is probably related to the creation of a proton-gradient across the plasma membrane and is generally distributed among yeasts.
在代表205种酵母菌种的248个菌株中，在无缓冲的细胞悬液中追踪了与添加葡萄糖相关的pH变化。葡萄糖引起外部培养基碱化，表明在34%的菌株中观察到H⁺-葡萄糖同向转运，其中大多数属于红酵母属、汉逊酵母属和假丝酵母属。仅在生长培养基中的葡萄糖耗尽后才观察到与葡萄糖转运相关的H⁺摄取。这一观察结果表明，一般来说，H⁺-葡萄糖同向转运体的合成受分解代谢物阻遏的控制。添加葡萄糖后，在大多数酵母（82%）中观察到酸化。这种能力可能与跨质膜质子梯度的形成有关，并且普遍存在于酵母中。
