Sugumar Mohanasundaram, Doss D Victor Arokia, Maddisetty P N Prasad
Research & Development Centre, Bharathiar University, Coimbatore, India.
Department of Biochemistry, PSG College of arts and Science, Coimbatore, India.
Integr Med Res. 2016 Dec;5(4):276-283. doi: 10.1016/j.imr.2016.06.005. Epub 2016 Jun 28.
Oxidative stress induced tissue damage might be the major cause for diabetes mellitus and its associated complications. The management of such oxidative stress is the biggest challenge over the decade. The main objective was to analyze the protective effect of ethanolic extract of L leaves on enzymatic and nonenzymatic antioxidant systems of hepatic and renal tissues in Streptozotocin-induced diabetes in rats.
The use of streptozotocin diabetes was induced in the experimental rats and the subsequent therapeutic effects of standard drug glibenclamide and L were compared. The levels of plasma insulin, glucose, urea, uric acid, creatinine, vitamin C, vitamin E, reduced glutathione, superoxide dismutase, catalase, glutathione peroxidase, and glutathione-s-tranferase were assayed in control and experimental groups of rats.
These alterations were detected throughout the study duration after the treatment with L and glibenclamide. A significant raise followed by the treatment with leaves in vitamin E, catalase, glutathione peroxidase and glutathione-s-tranferase was observed. It has been found that notable decline in the levels of vitamin C, reduced glutathione were observed in diabetic rats. The liver and kidney based antioxidant enzyme activities were significantly responsive to the treatment in diabetic rats. Apart from these antioxidant system, some vital changes were detected in the typical biochemical parameters such as level of protein, urea, uric acid, and creatinine from abnormal into normal in both the control and induced rats.
From the above said observations, it was very clear that, has helped to manage the oxidative tension in diabetic rats, which in turn may greatly support the hypoglycaemic potency of L.
氧化应激诱导的组织损伤可能是糖尿病及其相关并发症的主要原因。在过去十年中，应对这种氧化应激是最大的挑战。主要目的是分析L叶乙醇提取物对链脲佐菌素诱导的糖尿病大鼠肝组织和肾组织的酶促和非酶促抗氧化系统的保护作用。
在实验大鼠中诱导链脲佐菌素糖尿病，并比较标准药物格列本脲和L的后续治疗效果。在大鼠的对照组和实验组中测定血浆胰岛素、葡萄糖、尿素、尿酸、肌酐、维生素C、维生素E、还原型谷胱甘肽、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽 - s - 转移酶的水平。
在用L和格列本脲治疗后的整个研究期间都检测到了这些变化。观察到用L叶治疗后维生素E、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽 - s - 转移酶有显著升高。已发现糖尿病大鼠中维生素C、还原型谷胱甘肽水平显著下降。基于肝脏和肾脏的抗氧化酶活性对糖尿病大鼠的治疗有显著反应。除了这些抗氧化系统外，在对照组和诱导组大鼠中，一些典型的生化参数如蛋白质、尿素、尿酸和肌酐水平也从异常恢复到正常，出现了一些重要变化。
从上述观察结果很明显可以看出，L有助于控制糖尿病大鼠的氧化应激，这反过来可能极大地支持L的降血糖效力。
