Finerty S, Scullion F T, Morgan A J
Department of Pathology, University of Bristol, UK.
Clin Exp Immunol. 1988 Aug;73(2):181-5.
In the course of developing an effective Epstein-Barr (EB) virus vaccine, the immune responses in cotton-top tamarins to a tumourigenic dose of EB virus were studied. Cell mediated responses were measured using a tissue culture 'growth inhibition' assay where peripheral blood lymphocytes were tested for their ability to inhibit the outgrowth of autologous EB virus transformed lymphoblastoid cells. This system has previously been recognized as a very sensitive assay for detecting cell-mediated responses to EB virus in man. Using this assay no cell-mediated immunity was detected up to the time of death in two tamarins following injection with a tumourigenic dose of EB virus. However, two other animals which had recovered from tumours induced by a first dose of EB virus 18 months previously when subsequently re-stimulated with a second tumourigenic dose did exhibit cell-mediated responses. These latter animals remained healthy following the re-challenge and did not show evidence of EB virus-induced disease.
在研发一种有效的爱泼斯坦-巴尔（EB）病毒疫苗的过程中，研究了棉顶狨对致瘤剂量EB病毒的免疫反应。使用组织培养“生长抑制”试验来测量细胞介导的反应，在此试验中，检测外周血淋巴细胞抑制自体EB病毒转化的淋巴母细胞生长的能力。该系统先前已被公认为检测人类对EB病毒细胞介导反应的非常灵敏的试验。在两只棉顶狨注射致瘤剂量的EB病毒后直至死亡时，使用该试验未检测到细胞介导的免疫。然而，另外两只动物在18个月前因首次剂量的EB病毒诱导的肿瘤中恢复过来，当随后用第二次致瘤剂量再次刺激时，确实表现出细胞介导的反应。在再次攻击后，这些后一组动物保持健康，并且没有显示出EB病毒诱导疾病的迹象。
