Sanhal Cem Yasar, Daglar Korkut, Kara Ozgur, Yılmaz Zehra Vural, Turkmen Gulenay Gencosmanoglu, Erel Ozcan, Uygur Dilek, Yucel Aykan
a Department of Perinatology , Zekai Tahir Burak Women's Health Care, Training and Research Hospital , Ankara , Turkey.
b Department of Clinical Biochemistry, Faculty of Medicine , Yildirim Beyazit University , Ankara , Turkey.
J Matern Fetal Neonatal Med. 2018 Jun;31(11):1477-1482. doi: 10.1080/14767058.2017.1319922. Epub 2017 May 2.
The aim of our study was to evaluate the oxidative stress (OS) in pregnant women with intrahepatic cholestasis of pregnancy (ICP) by evaluating thiol/disulphide homeostasis using an alternative technique.
A total of 57 pregnant women with ICP were compared with 50 gestational age and body mass index matched controls. A recently defined method was used for the measurement of plasma native-total thiol and disulphide levels. The independent two-sample t test, Mann-Whitney-U test, Chi-square test, binary logistic regression with backward elimination and receiver operating characteristic (ROC) curve was performed for statistical analyses.
Pregnant women with ICP (n = 57) versus controls (n = 50) had significantly lower serum levels of native thiol (233.8 ± 47.4 μmol/L vs. 308.5 ± 51.7 μmol/L, p < .001), total thiol (258.4 ± 46.5 μmol/L vs. 328.0 ± 52.0 μmol/L, p < .001) and higher levels of disulphide (12.3 ± 3.6 μmol/L vs. 9.7 ± 3.4 μmol/L, p < .001). Binary logistic regression showed that the most important variables related to ICP were native thiol and total thiol. According to the ROC curve, the optimal cut-off level for native thiol was 280.0 μmol/L (sensitivity: 86%, specificity: 84.2%, area under the curve (AUC):0.896, 95% CI: 0.831-0.962, p < .001), and the optimal cut-off level for total thiol was 300.0 μmol/L (sensitivity: 86%, specificity: 80.7%, AUC: 0.883, 95% CI: 0.815-0.951, p < .001).
To our knowledge, this is the first study in the literature exploring thiol/disulphide balance in ICP. We found that thiol/disulphide balance indicate OS in pregnant woman with ICP.
我们研究的目的是通过使用一种替代技术评估硫醇/二硫化物稳态，来评估妊娠肝内胆汁淤积症（ICP）孕妇的氧化应激（OS）。
将57例ICP孕妇与50例孕周和体重指数相匹配的对照组进行比较。采用一种最近定义的方法来测量血浆天然总硫醇和二硫化物水平。进行独立两样本t检验、曼-惠特尼U检验、卡方检验、向后逐步淘汰的二元逻辑回归以及受试者工作特征（ROC）曲线分析进行统计分析。
ICP孕妇（n = 57）与对照组（n = 50）相比，血清天然硫醇水平显著降低（233.8±47.4μmol/L对308.5±51.7μmol/L，p <.001），总硫醇水平显著降低（258.4±46.5μmol/L对328.0±52.0μmol/L，p <.001），而二硫化物水平较高（12.3±3.6μmol/L对9.7±3.4μmol/L，p <.001）。二元逻辑回归显示，与ICP相关的最重要变量是天然硫醇和总硫醇。根据ROC曲线，天然硫醇的最佳截断水平为280.0μmol/L（敏感性：86%，特异性：84.2%，曲线下面积（AUC）：0.896，95%CI：0.831 - 0.962，p <.001），总硫醇的最佳截断水平为300.0μmol/L（敏感性：86%，特异性：80.7%，AUC：0.883，95%CI：0.815 - 0.951，p <.001）。
据我们所知，这是文献中第一项探索ICP中硫醇/二硫化物平衡的研究。我们发现硫醇/二硫化物平衡表明ICP孕妇存在氧化应激。
