Pearson G R
Department of Microbiology, Georgetown University Medical School, Washington, DC 20007.
J Virol Methods. 1988 Sep;21(1-4):97-104. doi: 10.1016/0166-0934(88)90056-0.
Monoclonal antibodies have now been prepared against a number of the major polypeptides associated with the different EBV-induced antigenic complexes. These antibodies have made it possible to purify and characterize most of these polypeptides and to identify the viral genes encoding for these proteins. In addition, new ELISA tests have been developed which measure antibodies in different immunoglobulin classes against the purified polypeptides. These assays have been useful in the diagnosis of EBV-associated diseases in clinical laboratories. The development of these antibodies and their application to the development of the new ELISA tests will be reviewed in this paper.
现已制备出针对多种与不同EB病毒诱导的抗原复合物相关的主要多肽的单克隆抗体。这些抗体使得纯化和鉴定这些多肽中的大多数成为可能，并能够识别编码这些蛋白质的病毒基因。此外，还开发了新的酶联免疫吸附测定（ELISA）试验，用于检测针对纯化多肽的不同免疫球蛋白类别的抗体。这些检测方法在临床实验室诊断EB病毒相关疾病中很有用。本文将综述这些抗体的研发及其在新型ELISA检测方法研发中的应用。
