Lei D J, Zhao G, Xie P, Li Y, Yuan H, Zou M, Niu J G, Ma X F
College of Fisheries, , , China.
, , China.
Genet Mol Res. 2017 May 4;16(2):gmr-16-02-gmr.16029376. doi: 10.4238/gmr16029376.
We used next-generation sequencing technology to characterize 19 genomic simple sequence repeat (SSR) markers and 11 expressed sequence tag (EST) SSR markers from Leuciscus leuciscus baicalensis, a small freshwater fish that is widely distributed in Xinjiang, China. Primers were used to test for polymorphisms in three L. leuciscus baicalensis populations in Xinjiang. There were 4-27 (average 11.3) alleles (N), the expected heterozygosity (H) was 0.36-0.94 (average 0.75 ± 0.14), the observed heterozygosity (H) was 0.37-1.00 (average 0.68 ± 0.18), and the polymorphism information content (PIC) was 0.31-0.93 (average 0.71). The averages of H and PIC for the EST-SSR markers were slightly lower than for the genomic SSR markers. Genetic analysis of the three populations showed similar results for PIC, H, and N. Amplifications were performed in nine other species; the top three transferability values were for Rutilus lacustris (80%), Leuciscus idus (76.7%), and Phoxinus ujmonensis (63.3%), with the following average values: PIC (0.56, 4.46, and 0.52); N (0.40, 3.00, and 0.32); and H (0.44, 2.74, and 0.22), respectively. L. leuciscus baicalensis is one of the most important commercial fish in Xinjiang, but in recent years, fishery resources have decreased sharply owing to water conservation projects, unreasonable utilization, and invasion by alien species. These novel SSR markers are appropriate for studies involving fingerprinting, gene flow, genetic diversity, population structure, and molecular-assisted breeding, and could contribute to the conservation of L. leuciscus baicalensis.
我们使用下一代测序技术对来自贝加尔雅罗鱼（Leuciscus leuciscus baicalensis）的19个基因组简单序列重复（SSR）标记和11个表达序列标签（EST）SSR标记进行了特征分析。贝加尔雅罗鱼是一种小型淡水鱼，在中国新疆广泛分布。使用引物对新疆的三个贝加尔雅罗鱼种群进行多态性检测。等位基因数（N）为4 - 27个（平均11.3个），期望杂合度（H）为0.36 - 0.94（平均0.75±0.14），观察杂合度（Ho）为0.37 - 1.00（平均0.68±0.18），多态性信息含量（PIC）为0.31 - 0.93（平均0.71）。EST - SSR标记的H和PIC平均值略低于基因组SSR标记。对这三个种群的遗传分析显示，PIC、H和N的结果相似。在其他九个物种中进行了扩增；转移率最高的三个物种是湖拟鲤（Rutilus lacustris，80%）、圆腹雅罗鱼（Leuciscus idus，76.7%）和乌苏里雅罗鱼（Phoxinus ujmonensis，63.3%），其平均值分别为：PIC（0.56、0.46和0.52）；N（0.40、3.00和0.32）；H（0.44、0.74和0.22）。贝加尔雅罗鱼是新疆最重要的商业鱼类之一，但近年来，由于节水工程、不合理利用以及外来物种入侵，渔业资源急剧减少。这些新型SSR标记适用于指纹识别、基因流、遗传多样性、种群结构和分子辅助育种等研究，并有助于贝加尔雅罗鱼的保护。
