Abd El-Aziz A M, El Backly Rania M, Taha Nahla A, El-Maghraby Azza, Kandil Sherif H
Department of Fabrication Technology, Advanced Technology and New Materials Research Institute, City for Scientific Research and Technology Applications, Alexandria, Egypt; Department of Materials Science, Institute of Graduate Studies and Research, Alexandria University, Egypt.
Department of Endodontics and Tissue Engineering Laboratories, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
Mater Sci Eng C Mater Biol Appl. 2017 Jul 1;76:1188-1195. doi: 10.1016/j.msec.2017.02.053. Epub 2017 Mar 1.
Critical size bone defects are orthopedic defects that will not heal without intervention or that will not completely heal over the natural life time of the animal. Although bone generally has the ability to regenerate completely however, critical defects require some sort of scaffold to do so. In the current study we proposed a method to obtain a carbon nanofibrous/Hydroxyapatite (HA) bioactive scaffold. The carbon nanofibrous (CNF) nonwoven fabrics were obtained by the use of the electrospinning process of the polymeric solution of poly acrylonitrile "PAN" and subsequent stabilization and carbonization processes. The CNFs sheets were functionalized by both hydroxyapatite (HA) and bovine serum albumin (BSA). The HA was added to the electrospun solution, but in case of (BSA), it was adsorbed after the carbonization process. The changes in the properties taking place in the precursor sheets were investigated using the characterization methods (SEM, FT-IR, TGA and EDX). The prepared materials were tested for biocompatibility via subcutaneous implantation in New Zealand white rabbits. We successfully prepared biocompatible functionalized sheets, which have been modified with HA or HA and BSA. The sheets that were functionalized by both HA and BSA are more biocompatible with fewer inflammatory cells of (neutrophils and lymphocytes) than ones with only HA over the period of 3weeks.
临界尺寸骨缺损是指那些不经干预就无法愈合或在动物自然寿命期间无法完全愈合的骨科缺损。虽然一般来说骨具有完全再生的能力，然而，临界缺损需要某种支架才能实现再生。在本研究中，我们提出了一种制备碳纳米纤维/羟基磷灰石（HA）生物活性支架的方法。碳纳米纤维（CNF）无纺布是通过对聚丙烯腈（PAN）聚合物溶液进行静电纺丝工艺，随后经过稳定化和碳化工艺制备而成。CNF片材通过羟基磷灰石（HA）和牛血清白蛋白（BSA）进行功能化处理。HA被添加到静电纺丝溶液中，但对于BSA，它是在碳化过程后吸附的。使用表征方法（扫描电子显微镜、傅里叶变换红外光谱、热重分析和能谱分析）研究前驱体片材中发生的性能变化。通过在新西兰白兔皮下植入对制备的材料进行生物相容性测试。我们成功制备了经HA或HA与BSA修饰的具有生物相容性的功能化片材。在3周的时间里，与仅用HA功能化处理的片材相比，同时用HA和BSA功能化处理的片材具有更好的生物相容性，炎症细胞（中性粒细胞和淋巴细胞）更少。
