Department of Internal Medicine, General Hospital Slovenj Gradec, Slovenj Gradec, Slovenia.
Bosn J Basic Med Sci. 2017 Aug 20;17(3):262-267. doi: 10.17305/bjbms.2017.1823.
Gene polymorphisms associated with the renin-angiotensin-aldosterone system (RAAS) have been extensively studied in diabetic nephropathy (DN) patients, due to therapeutic potential of targeting the RAAS and slowing down the disease progression. The aim of our study was to examine the association between angiotensinogen (AGT) gene polymorphisms (rs699 and rs4762) and DN in Caucasians with type 2 diabetes mellitus (T2DM). A total of 651 unrelated Slovenian (Caucasian) T2DM patients were tested for AGT rs699 and rs4762 polymorphisms using a novel fluorescence-based kompetitive allele-specific polymerase chain reaction (KASPar) assay. A study group consisted of 276 T2DM patients with DN, while control group included 375 patients without DN but who have had T2DM for >10 years. For rs699 polymorphism, the frequencies of GG, GA and AA genotypes were 20.6%, 52.2% and 27.2%, respectively in T2DM patients and 23.4%, 48.1% and 28.5%, respectively in controls. The distributions of GG, GA and AA genotypes for rs4762 polymorphism were 73.9%, 23.2% and 2.9%, respectively in T2DM patients and 70.4%, 27.5% and 2.1%, respectively in controls. No significant differences in the allele frequencies were found between T2DM patients and controls for both polymorphisms. AGT rs699 and rs4762 missense polymorphisms are not associated with DN in our subset of Slovenian T2DM patients.
血管紧张素原（AGT）基因多态性（rs699 和 rs4762）与 2 型糖尿病肾病（DN）的相关性在糖尿病患者中进行了广泛研究，因为靶向肾素-血管紧张素-醛固酮系统（RAAS）和减缓疾病进展具有治疗潜力。我们的研究目的是检验血管紧张素原（AGT）基因多态性（rs699 和 rs4762）与斯洛文尼亚（白种人）2 型糖尿病患者 DN 的相关性。使用新型荧光竞争等位基因特异性聚合酶链反应（KASPar）检测 651 例无关斯洛文尼亚 2 型糖尿病患者的 AGT rs699 和 rs4762 多态性。研究组由 276 例 2 型糖尿病合并 DN 的患者组成，对照组包括 375 例无 DN 但患 2 型糖尿病>10 年的患者。对于 rs699 多态性，2 型糖尿病患者 GG、GA 和 AA 基因型的频率分别为 20.6%、52.2%和 27.2%，对照组分别为 23.4%、48.1%和 28.5%。rs4762 多态性 GG、GA 和 AA 基因型的分布在 2 型糖尿病患者中分别为 73.9%、23.2%和 2.9%，在对照组中分别为 70.4%、27.5%和 2.1%。两种多态性在 2 型糖尿病患者和对照组之间的等位基因频率无显著差异。在我们的斯洛文尼亚 2 型糖尿病患者亚组中，AGT rs699 和 rs4762 错义多态性与 DN 无关。
