Campbell J B, Crocker J, Shenoi P M
Department of Otolaryngology, East Birmingham Hospital.
J Laryngol Otol. 1988 Oct;102(10):905-8. doi: 10.1017/s0022215100106784.
The clinician is almost entirely dependent on the histopathologist to accurately diagnose minor salivary gland tumours, but in some cases the histological interpretation of the specimen is very difficult. Recently it has been demonstrated using immunohistochemical techniques that S-100 protein is present in some salivary gland tissues and its localization has been used as an aid in the differentiation of major salivary gland tumours. To assess its value in the diagnosis of minor salivary gland tumours it was localized in sections from 15 such tumours using both a standard peroxidase-antiperoxidase (PAP) and a newly developed immunogold-silver staining sequence (IGSS) technique. Strong staining for S-100 protein was seen in the nuclei and cytoplasm of the cellular areas and also in the cells in the chondroid and myxoid areas of pleomorphic adenomas. Generally the staining was more intense and widespread with the IGSS method. No staining was observed in any of the other tumour types. We conclude that S-100 protein localization is a valuable aid in the differentiation of minor salivary gland tumours. Furthermore, the IGSS method enables more sensitive 'reading' of the staining reaction.
临床医生几乎完全依赖组织病理学家来准确诊断小唾液腺肿瘤，但在某些情况下，对标本进行组织学解释非常困难。最近通过免疫组织化学技术已经证明，S-100蛋白存在于一些唾液腺组织中，其定位已被用于辅助诊断大唾液腺肿瘤。为了评估其在诊断小唾液腺肿瘤中的价值，使用标准的过氧化物酶-抗过氧化物酶（PAP）和新开发的免疫金银染色序列（IGSS）技术，对15例此类肿瘤的切片进行了S-100蛋白的定位。在多形性腺瘤的细胞区域的细胞核和细胞质以及软骨样和黏液样区域的细胞中均可见到S-100蛋白的强染色。一般来说，IGSS方法的染色更强烈且更广泛。在任何其他肿瘤类型中均未观察到染色。我们得出结论，S-100蛋白定位在小唾液腺肿瘤的鉴别诊断中是一种有价值的辅助手段。此外，IGSS方法能够更灵敏地“读取”染色反应。
