Prasad Andhare A, Babu Subramanian
School of Bio Sciences and Technology, VIT University, Vellore 632014, India.
An Acad Bras Cienc. 2017 Apr-Jun;89(2):1027-1040. doi: 10.1590/0001-3765201720160617. Epub 2017 May 2.
We attempted to study the compatibility among plant beneficial bacteria in the culture level by growing them near in the nutrient agar plates. Among all the bacteria tested, Rhizobium was found to inhibit the growth of other bacteria. From the compatible group of PGPR, we have selected one biofertilizer (Azospirillum brasilense strain TNAU) and one biocontrol agent (Pseudomonas fluorescens strain PF1) for further studies in the pot culture. We have also developed a bioformulation which is talc powder based, for individual bacteria and mixed culture. This formulation was used as seed treatment, soil application, seedling root dip and foliar spray in groundnut crop in vitro germination conditions. A. brasilense was found to enhance the tap root growth and P. fluorescens, the lateral root growth. The other growth parameters like shoot growth, number of leaves were enhanced by the combination of both of the bacteria than their individual formulations. Among the method of application tested in our study, soil application was found to be the best in yielding better results of plant growth promotion.
我们试图通过在营养琼脂平板上使植物有益细菌彼此靠近生长，在培养水平上研究它们之间的兼容性。在所有测试的细菌中，发现根瘤菌会抑制其他细菌的生长。从植物根际促生细菌的兼容组中，我们选择了一种生物肥料（巴西固氮螺菌TNAU菌株）和一种生物防治剂（荧光假单胞菌PF1菌株）用于盆栽培养的进一步研究。我们还开发了一种以滑石粉为基础的生物制剂，用于单一细菌和混合培养。该制剂在花生作物的体外萌发条件下用作种子处理、土壤施用、幼苗根浸和叶面喷施。发现巴西固氮螺菌可促进主根生长，荧光假单胞菌可促进侧根生长。与它们各自的制剂相比，两种细菌的组合对其他生长参数如地上部生长、叶片数量的促进作用更强。在我们研究测试的施用方法中，发现土壤施用在促进植物生长方面效果最佳。
