Al-Mekhlafi Fahd A, Abutaha Nael, Mashaly Ashraf M A, Nasr Fahd A, Ibrahim Khalid E, Wadaan Mohamed A
Bioproducts Research Chair, Department of Zoology, College of Science, King Saud University, Saudi Arabia.
Department of Agricultural Production, College of Agriculture and Veterinary Medicine, Thamar University, Yemen.
Saudi J Biol Sci. 2017 May;24(4):817-821. doi: 10.1016/j.sjbs.2016.07.003. Epub 2016 Aug 9.
Effects of methanol extracts of on different cancer cell lines and on the mortality rates of (Diptera: Culicidae) were investigated. Among the cell lines tested, the Jurkat cell line was the most sensitive to the methanol extract and ethyl acetate fraction, with reported LC values of 50.18 and 48.73 μg/ml respectively. Conversely, methanol extracts were not that toxic to the A549 cell line though the toxicity increased on further purification. The percentage of growth inhibition was dose dependent for the methanol extract and ethyl acetate fraction. The ethyl acetate fraction showed higher toxicity to all cell lines tested when compared to the methanol extract. The results showed that methanol extracts of plant seeds caused 100% mortality of mosquito larvae at a concentration of 1000 μg/ml after 24 h of treatment. The LC and LC values of were found to be 531.07 and 905.95 μg/ml against and 502.32 and 867.63 μg/ml against respectively. From the investigations, it was concluded that the crude extract of showed a weak potential for controlling the larval instars of and . However, on further purification the extract lost the larvicidal activity. The ethyl acetate fraction showed higher toxicity to all cell lines tested when compared to the methanol extract. The ethyl acetate fraction investigated in this study appears to have a weak larvicidal activity but a promising cytotoxic activity. Future studies will include purification and investigation in further detail of the action of on Cancer Cell Lines and mosquitoes.
研究了[植物名称]甲醇提取物对不同癌细胞系以及对[蚊虫名称]（双翅目：蚊科）死亡率的影响。在所测试的细胞系中，Jurkat细胞系对甲醇提取物和乙酸乙酯馏分最为敏感，报道的LC₅₀值分别为50.18和48.73μg/ml。相反，甲醇提取物对A549细胞系的毒性不强，不过进一步纯化后毒性增加。甲醇提取物和乙酸乙酯馏分的生长抑制百分比呈剂量依赖性。与甲醇提取物相比，乙酸乙酯馏分对所有测试细胞系显示出更高的毒性。结果表明，植物种子的甲醇提取物在处理24小时后，浓度为1000μg/ml时可导致蚊虫幼虫100%死亡。发现[植物名称]对[蚊虫名称1]的LC₅₀和LC₉₀值分别为531.07和905.95μg/ml，对[蚊虫名称2]分别为502.32和867.63μg/ml。从这些研究得出结论，[植物名称]的粗提物在控制[蚊虫名称1]和[蚊虫名称2]幼虫龄期方面显示出较弱的潜力。然而，进一步纯化后提取物失去了杀幼虫活性。与甲醇提取物相比，乙酸乙酯馏分对所有测试细胞系显示出更高的毒性。本研究中所研究的乙酸乙酯馏分似乎具有较弱的杀幼虫活性，但具有有前景的细胞毒性活性。未来的研究将包括对[植物名称]对癌细胞系和蚊虫作用的进一步纯化和详细研究。
