Biological activity of seed extracts on different cancer cell lines and , (Diptera: Culicidae).

Effects of methanol extracts of on different cancer cell lines and on the mortality rates of (Diptera: Culicidae) were investigated. Among the cell lines tested, the Jurkat cell line was the most sensitive to the methanol extract and ethyl acetate fraction, with reported LC values of 50.18 and 48.73 μg/ml respectively. Conversely, methanol extracts were not that toxic to the A549 cell line though the toxicity increased on further purification. The percentage of growth inhibition was dose dependent for the methanol extract and ethyl acetate fraction. The ethyl acetate fraction showed higher toxicity to all cell lines tested when compared to the methanol extract. The results showed that methanol extracts of plant seeds caused 100% mortality of mosquito larvae at a concentration of 1000 μg/ml after 24 h of treatment. The LC and LC values of were found to be 531.07 and 905.95 μg/ml against and 502.32 and 867.63 μg/ml against respectively. From the investigations, it was concluded that the crude extract of showed a weak potential for controlling the larval instars of and . However, on further purification the extract lost the larvicidal activity. The ethyl acetate fraction showed higher toxicity to all cell lines tested when compared to the methanol extract. The ethyl acetate fraction investigated in this study appears to have a weak larvicidal activity but a promising cytotoxic activity. Future studies will include purification and investigation in further detail of the action of on Cancer Cell Lines and mosquitoes.