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喀麦隆的[具体植物名称1]、[具体植物名称2]、[具体植物名称3]以及其他三种植物的甲醇提取物对多重耐药革兰氏阴性菌的抗菌活性。

Antibacterial activities of the methanol extracts of , , and three other Cameroonian plants against multi-drug resistant Gram-negative bacteria.

作者信息

Tchinda Cedric F, Voukeng Igor K, Beng Veronique P, Kuete Victor

机构信息

Department of Biochemistry, Faculty of Science, University of Dschang, Cameroon.

出版信息

Saudi J Biol Sci. 2017 May;24(4):950-955. doi: 10.1016/j.sjbs.2016.01.033. Epub 2016 Jan 25.

Abstract

In the last 10 years, resistance in Gram-negative bacteria has been increasing. The present study was designed to evaluate the antibacterial activities of the methanol extracts of six Cameroonian medicinal plants , , , , and against a panel of 15 multidrug resistant Gram-negative bacterial strains. The broth microdilution was used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the extracts. The preliminary phytochemical screening of the extracts was conducted according to the reference qualitative phytochemical methods. Results showed that all extracts contained compounds belonging to the classes of polyphenols and triterpenes, other classes of chemicals being selectively distributed. The best antibacterial activities were recorded with bark and root extracts of as well as with extract, with MIC values ranging from 64 to 1024 μg/mL on 93.3% of the fifteen tested bacteria. The lowest MIC value of 64 μg/mL was recorded with bark extract against EA289. Finally, the results of this study provide evidence of the antibacterial activity of the tested plants and suggest their possible use in the control of multidrug resistant phenotypes.

摘要

在过去10年中,革兰氏阴性菌的耐药性一直在增加。本研究旨在评估六种喀麦隆药用植物、、、、和的甲醇提取物对一组15种多重耐药革兰氏阴性菌菌株的抗菌活性。采用肉汤微量稀释法测定提取物的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。根据参考定性植物化学方法对提取物进行初步植物化学筛选。结果表明,所有提取物均含有多酚和三萜类化合物,其他化学类别则选择性分布。树皮和根提取物以及提取物的抗菌活性最佳,在15种受试细菌中的93.3%上,MIC值范围为64至1024μg/mL。树皮提取物对EA289的最低MIC值为64μg/mL。最后,本研究结果提供了受试植物抗菌活性的证据,并表明它们可能用于控制多重耐药表型。

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