Howlader Md Sariful Islam, Siraj Md Afjalus, Dey Shubhra Kanti, Hira Arpona, Ahmed Arif, Hossain Md Hemayet
Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh.
Department of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, Hilo, HI 96720, USA.
Evid Based Complement Alternat Med. 2017;2017:7390359. doi: 10.1155/2017/7390359. Epub 2017 Apr 12.
is traditionally used in the ailment of pain, inflammation, and neurological disorders. The present study set out to evaluate the in vivo antinociceptive, anti-inflammatory, and sedative activity of the ethanol extract of bark (EFHB). The antinociceptive activity of EFHB was evaluated by using acetic acid induced writhing, formalin, hot plate, and tail immersion methods in Swiss albino mice. Its anti-inflammatory activity was assessed by using carrageenan and histamine induced rat paw oedema test in Wister rats. The central stimulating activity was studied by using pentobarbital induced hypnosis, hole cross, and open field tests in Swiss albino mice. EFHB demonstrated antinociceptive activity both centrally and peripherally. It showed 62.24% of writhing inhibition. It significantly inhibited licking responses in early (59.29%) and late phase (71.61%). It increased the reaction time to the thermal stimulus in both hot plate and tail immersion. It inhibited the inflammation to the extent of 59.49%. A substantial increase in duration of sleep up to 60.80 min and decrease of locomotion up to 21.70 at 400 mg/kg were also observed. We found significant dose dependent antinociceptive, anti-inflammatory, and sedative properties of EFHB in experimental animal models.
传统上用于治疗疼痛、炎症和神经紊乱疾病。本研究旨在评估树皮乙醇提取物（EFHB）的体内抗伤害感受、抗炎和镇静活性。通过在瑞士白化小鼠中使用醋酸诱导扭体、福尔马林、热板和尾浸法评估EFHB的抗伤害感受活性。通过在Wistar大鼠中使用角叉菜胶和组胺诱导的大鼠爪肿胀试验评估其抗炎活性。通过在瑞士白化小鼠中使用戊巴比妥诱导的催眠、穿洞和旷场试验研究其中枢刺激活性。EFHB在中枢和外周均表现出抗伤害感受活性。它显示出62.24%的扭体抑制率。它显著抑制早期（59.29%）和晚期（71.61%）的舔舐反应。它增加了热板和尾浸试验中对热刺激的反应时间。它抑制炎症的程度达59.49%。在400mg/kg剂量下还观察到睡眠时间显著增加至60.80分钟，运动能力下降至21.70。我们发现在实验动物模型中EFHB具有显著的剂量依赖性抗伤害感受、抗炎和镇静特性。
