Ardiel Evan L, Kumar Abhishek, Marbach Joseph, Christensen Ryan, Gupta Rishi, Duncan William, Daniels Jonathan S, Stuurman Nico, Colón-Ramos Daniel, Shroff Hari
Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland; Grass Lab, Marine Biological Laboratories, Woods Hole, Massachusetts.
Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland; Grass Lab, Marine Biological Laboratories, Woods Hole, Massachusetts.
Biophys J. 2017 May 9;112(9):1975-1983. doi: 10.1016/j.bpj.2017.02.035.
The lack of physiological recordings from Caenorhabditis elegans embryos stands in stark contrast to the comprehensive anatomical and gene expression datasets already available. Using light-sheet fluorescence microscopy to address the challenges associated with functional imaging at this developmental stage, we recorded calcium dynamics in muscles and neurons and developed analysis strategies to relate activity and movement. In muscles, we found that the initiation of twitching was associated with a spreading calcium wave in a dorsal muscle bundle. Correlated activity in muscle bundles was linked with early twitching and eventual coordinated movement. To identify neuronal correlates of behavior, we monitored brainwide activity with subcellular resolution and identified a particularly active cell associated with muscle contractions. Finally, imaging neurons of a well-defined adult motor circuit, we found that reversals in the eggshell correlated with calcium transients in AVA interneurons.
秀丽隐杆线虫胚胎缺乏生理记录，这与现有的全面解剖学和基因表达数据集形成了鲜明对比。我们使用光片荧光显微镜来应对这一发育阶段功能成像相关的挑战，记录了肌肉和神经元中的钙动力学，并开发了分析策略来关联活动和运动。在肌肉中，我们发现抽搐的起始与背侧肌肉束中传播的钙波有关。肌肉束中的相关活动与早期抽搐和最终的协调运动相关联。为了识别行为的神经元关联，我们以亚细胞分辨率监测全脑活动，并识别出一个与肌肉收缩相关的特别活跃的细胞。最后，对一个明确的成年运动回路的神经元进行成像，我们发现卵壳中的反转与AVA中间神经元中的钙瞬变相关。
