Laboratoire de Physique Statistique de l'Ecole Normale Supérieure, CNRS, UPMC, 24, rue Lhomond, 75005 Paris, France.
J Neurosci Methods. 2010 Mar 30;187(2):229-34. doi: 10.1016/j.jneumeth.2010.01.011. Epub 2010 Jan 21.
In order to understand how neuronal circuits control locomotory patterns it is necessary to record neuronal activity of freely behaving animals. Here, using a new automated system for simultaneous recording of behavior and neuronal activity in freely moving Caenorhabditis elegans on standard agar plates, we show that spontaneous reversals from forward to backward locomotion reflect precisely the activity of the AVA command interneurons. We also witness spontaneous activity transients in the PLM sensory neurons during free behavior of the worm in standard conditions. We show that these activity transients are coupled to short spontaneous forward accelerations of the worm.
为了理解神经元回路如何控制运动模式，有必要记录自由活动动物的神经元活动。在这里，我们使用一种新的自动化系统，在标准琼脂平板上对自由移动的秀丽隐杆线虫的行为和神经元活动进行同时记录，结果表明，从向前运动到向后运动的自发反转精确地反映了 AVA 指令中间神经元的活动。我们还在线虫在标准条件下自由活动时，观察到 PLM 感觉神经元中自发活动的瞬变。我们表明，这些活动瞬变与线虫的短暂自发向前加速有关。
