Odds F C, Webster C E
Department of Microbiology, University of Leicester, UK.
J Antimicrob Chemother. 1988 Oct;22(4):473-81. doi: 10.1093/jac/22.4.473.
Clotrimazole, fluconazole, itraconazole and ketoconazole were tested for their influence on three aspects of host/parasite interactions in the context of Candida infections. None of clotrimazole, itraconazole or ketoconazole had any effect on the adherence of C. albicans to vaginal epithelial cells, in vitro, regardless of whether the drugs were used to pretreat the fungi or the vaginal cells or were added to the fungus/vaginal cell mixture. Clotrimazole pretreatment of polymorphonuclear leucocytes led to a marked suppression of their ability to phagocytose and kill C. albicans, but fluconazole and ketoconazole had no similar effect. None of these three antifungals affected phagocytosis or killing when they were added to the leucocyte candida mixture or when used to pretreat the fungi. Clotrimazole and ketoconazole both reduced proliferative responses of lymphocyte suspensions to mitogens but fluconazole showed no anti-lymphocyte effect. This difference in action against lymphocytes may help to explain the known disparity between the antifungal effects of fluconazole and ketoconazole against C. albicans in vivo and against experimental candida infections in vitro. Clotrimazole effected a suppression of ATP concentrations in lymphocytes but fluconazole and ketoconazole had no similar effects.
在念珠菌感染的背景下，对克霉唑、氟康唑、伊曲康唑和酮康唑在宿主/寄生虫相互作用的三个方面的影响进行了测试。在体外，无论这些药物是用于预处理真菌或阴道细胞，还是添加到真菌/阴道细胞混合物中，克霉唑、伊曲康唑或酮康唑均对白色念珠菌黏附阴道上皮细胞没有任何影响。对多形核白细胞进行克霉唑预处理会导致其吞噬和杀死白色念珠菌的能力显著受到抑制，但氟康唑和酮康唑没有类似作用。当将这三种抗真菌药物添加到白细胞-念珠菌混合物中或用于预处理真菌时，它们均不影响吞噬作用或杀伤作用。克霉唑和酮康唑均降低了淋巴细胞悬液对有丝分裂原的增殖反应，但氟康唑没有抗淋巴细胞作用。这种对淋巴细胞作用的差异可能有助于解释氟康唑和酮康唑在体内对白色念珠菌的抗真菌作用以及在体外对实验性念珠菌感染的抗真菌作用之间已知的差异。克霉唑会导致淋巴细胞中ATP浓度降低，但氟康唑和酮康唑没有类似作用。
