Roshanravan Neda, Asgharian Parina, Dariushnejad Hassan, Mesri Alamdari Naimeh, Mansoori Behzad, Mohammadi Ali, Alipour Shahriar, Barati Meisam, Ghavami Abed, Ghorbanzadeh Vajihe, Aamazadeh Fatemeh, Ostadrahimi Alireza
Cardiovascular Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Adv Pharm Bull. 2018 Nov;8(4):667-674. doi: 10.15171/apb.2018.075. Epub 2018 Nov 29.
Pancreatic adenocarcinoma has a high prevalence all over the world. Most of the therapeutic approaches failed as a result of tumor invasion and rapid metastasis. Several natural plants have been shown to have promising therapeutic effects. Thus, the aim of this study was to investigate the cytotoxic activity of Eryngium billardieri against PANC-1 cancer cell lines. Dimethylthiazole diphenyltetrazolium bromide assay (MTT assay) and flow cytometry were used to assess the cytotoxicity of E. billardieri extracts against PANC-1 cancer cell lines. Quantitative Polymerase Chain Reaction (qPCR) was conducted to investigate the expression levels of Bcl2- associated X protein (BAX) and cyclin D1. The results of the MTT assay showed that E. billardieri extracts had cytotoxic effects on PANC- 1 cancer cell lines. Moreover, the findings from the gene expression confirmed the over expression of Bax, and under expression of cyclin D1 following treatment with dichloromethane (DCM) and n-hexane (n- hex) extracts in cancer cells (P < 0.05). Interestingly, the flow cytometry results showed that DCM and n- hex extracts of E. billardieri induced apoptosis in PANC- 1 cancer cell lines. The results of this study demonstrated that DCM and n- hex extracts of E. billardieri significantly induce apoptosis by increasing Bax and decreasing cyclin D1 mRNA expression. Therefore, E. billardieri may be regarded as a novel approach for treatment of pancreatic cancer as a result of its promising apoptotic and cytotoxic properties.
胰腺腺癌在全球范围内具有较高的发病率。由于肿瘤侵袭和快速转移，大多数治疗方法都失败了。几种天然植物已被证明具有良好的治疗效果。因此，本研究的目的是研究刺芹对PANC - 1癌细胞系的细胞毒性活性。采用噻唑蓝比色法（MTT法）和流式细胞术评估刺芹提取物对PANC - 1癌细胞系的细胞毒性。进行定量聚合酶链反应（qPCR）以研究Bcl2相关X蛋白（BAX）和细胞周期蛋白D1的表达水平。MTT法结果表明，刺芹提取物对PANC - 1癌细胞系具有细胞毒性作用。此外，基因表达结果证实，用二氯甲烷（DCM）和正己烷（n - hex）提取物处理癌细胞后，Bax表达上调，细胞周期蛋白D1表达下调（P < 0.05）。有趣的是，流式细胞术结果表明，刺芹的DCM和n - hex提取物可诱导PANC - 1癌细胞系凋亡。本研究结果表明，刺芹的DCM和n - hex提取物通过增加Bax表达和降低细胞周期蛋白D1 mRNA表达显著诱导凋亡。因此，由于其有前景的凋亡和细胞毒性特性，刺芹可被视为一种治疗胰腺癌的新方法。
