Khati Priyanka, Chaudhary Parul, Gangola Saurabh, Bhatt Pankaj, Sharma Anita
GB Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India.
3 Biotech. 2017 May;7(1):81. doi: 10.1007/s13205-017-0668-y. Epub 2017 May 12.
The present study evaluated the effect of nanochitosan in combination with plant growth promoting rhizobacteria (PGPR), PS2 and PS10 on maize growth. The PGPR were earlier recognized as Bacillus spp. on the basis of 16S rDNA sequencing. The observation revealed enhanced plant health parameters like seed germination (from 60 to 96.97%), plant height (1.5-fold increase), and leaf area (twofold). Variability in different physicochemical parameters (pH, oxidizable organic carbon, available phosphorous, available potassium, ammoniacal nitrogen and nitrate nitrogen) was observed. Activities of soil health indicator enzymes (dehydrogenase, fluorescein diacetate hydrolysis and alkaline phosphatase) were also enhanced 2 to 3 fold. Plant metabolites with respect to different treatments were also analyzed using gas chromatography-mass spectroscopy (GC-MS) and the result revealed an increase in the amounts of alcohols, acid ester and aldehyde compounds. Increase in organic acids indicates increased stress tolerance mechanism operating in maize plant after treatment of nanochitosan.
本研究评估了纳米壳聚糖与植物促生根际细菌(PGPR)PS2和PS10组合对玉米生长的影响。基于16S rDNA测序,这些PGPR先前被鉴定为芽孢杆菌属。观察结果显示,植物健康参数得到改善,如种子发芽率(从60%提高到96.97%)、株高(增加了1.5倍)和叶面积(增加了两倍)。观察到不同理化参数(pH值、可氧化有机碳、有效磷、有效钾、氨态氮和硝态氮)存在差异。土壤健康指标酶(脱氢酶、荧光素二乙酸酯水解酶和碱性磷酸酶)的活性也提高了2至3倍。还使用气相色谱-质谱联用仪(GC-MS)分析了不同处理下的植物代谢产物,结果显示醇类、酸酯类和醛类化合物的含量有所增加。有机酸含量的增加表明,纳米壳聚糖处理后玉米植株的胁迫耐受机制增强。