Gnyawali Vaskar, Saremi Mohammadali, Kolios Michael C, Tsai Scott S H
Biomicrofluidics. 2017 May 4;11(3):034104. doi: 10.1063/1.4983147. eCollection 2017 May.
We present a simple technique to generate stable hydrodynamically focused flows by driving the flow with hydrostatic pressure from liquid columns connected to the inlets of a microfluidic device. Importantly, we compare the focused flows generated by hydrostatic pressure and classical syringe pump driven flows and find that the stability of the hydrostatic pressure driven technique is significantly better than the stability achieved via syringe pumps, providing fluctuation-free focused flows that are suitable for sensitive microfluidic flow cytometry applications. We show that the degree of flow focusing with the hydrostatic method can be accurately controlled by the simple tuning of the liquid column heights. We anticipate that this approach to stable flow focusing will find many applications in microfluidic cytometry technologies.
我们提出了一种简单的技术，通过利用连接到微流控设备入口的液柱产生的静水压力来驱动流体，从而生成稳定的流体动力学聚焦流。重要的是，我们比较了由静水压力产生的聚焦流和传统注射泵驱动的流，发现静水压力驱动技术的稳定性明显优于注射泵实现的稳定性，能提供无波动的聚焦流，适用于灵敏的微流控流式细胞术应用。我们表明，通过简单调整液柱高度，就可以精确控制静水压力法的流聚焦程度。我们预计，这种实现稳定流聚焦的方法将在微流控细胞术技术中得到广泛应用。
