Kim Ki-Jung, Choi Sunkyung, Sang Cho Yong, Yang Seok-Jo, Cho Young-Sam, Kim Kee K
Department of Mechatronics Engineering, College of Engineering, Chungnam National University, Daejeon, 34134, Republic of Korea.
Department of Biochemistry, College of Natural Sciences, Chungnam National University, Daejeon, 34134, Republic of Korea.
J Mater Sci Mater Med. 2017 Jun;28(6):96. doi: 10.1007/s10856-017-5908-5. Epub 2017 May 16.
Magnesium (Mg) ions are the most abundant intracellular divalent cations and play a pivotal role in numerous cellular processes. Biodegradable Mg-containing materials, including scaffolds, are promising candidates for orthopedic applications. Here, we investigated the effect of Mg ions on the cellular properties of osteoblasts. Cytotoxicity tests on osteoblasts confirmed that no cytotoxic effects were found up to a supplementing Mg ion concentration of 10 mM. Mg ions at a concentration of 5 mM increased the migration and invasiveness of osteoblasts. To investigate the stimulatory effect of Mg ions on cell motility in scaffolds, we fabricated 10 wt% Mg ion-containing polycaprolactone (PCL) scaffolds, using the wire-network molding (WNM) technique. Mg ion-containing scaffolds persistently released Mg ions at a concentration of 5 mM in the media after pre-incubation. Furthermore, increased cell motility was confirmed in Mg ion-containing scaffolds by quantification of genomic DNA and protein content. Our results provide an important basis for the function of Mg ions and their effect on cell motility, and propose a novel role for Mg ions in scaffold applications.
镁（Mg）离子是细胞内含量最丰富的二价阳离子，在众多细胞过程中发挥着关键作用。包括支架在内的可生物降解含镁材料是骨科应用中有前景的候选材料。在此，我们研究了镁离子对成骨细胞细胞特性的影响。对成骨细胞的细胞毒性测试证实，在补充镁离子浓度达到10 mM之前未发现细胞毒性作用。浓度为5 mM的镁离子增加了成骨细胞的迁移和侵袭能力。为了研究镁离子对支架中细胞运动性的刺激作用，我们使用丝网成型（WNM）技术制备了含10 wt%镁离子的聚己内酯（PCL）支架。预孵育后，含镁离子的支架在培养基中持续释放浓度为5 mM的镁离子。此外，通过对基因组DNA和蛋白质含量的定量分析，证实含镁离子的支架中细胞运动性增加。我们的结果为镁离子的功能及其对细胞运动性的影响提供了重要依据，并提出了镁离子在支架应用中的新作用。
