Panieri Emiliano, Santoro Massimo M
Department of Molecular Biotechnology and Health Sciences, University of Turin, Turin, Italy.
Data Brief. 2017 Apr 27;12:427-432. doi: 10.1016/j.dib.2017.04.034. eCollection 2017 Jun.
In this article we used transgenic zebrafish lines that express compartment-specific isoforms of the roGFP2-Orp1 and Grx1-roGFP2 biosensors, described in Panieri et al (2017) [1], to test the contribute of the pentose phosphate pathway and of the glutathione biosynthesis in the antioxidant capacity of myocardial and endothelial cells in vivo. The transgenic zebrafish embryos were subdued to metabolic inhibition and subsequently challenged with H2O2 or the redox-cycling agent menadione to respectively mimic acute or chronic oxidative stress. Confocal time-lapse recordings were performed to follow the compartmentalized HO and E changes in the cardiovascular tissues of zebrafish embryos at 48 h post fertilization. After sequential excitation at 405 nm and 488 nm the emission was collected between 500-520 nm every 2 min for an overall duration of 60 min. The 405/488 nm ratio was normalized to the initial value obtained before oxidants addition and plotted over time. The analysis and the interpretation of the data can be found in the associated article [1].
在本文中，我们使用了转基因斑马鱼品系，这些品系表达了roGFP2-Orp1和Grx1-roGFP2生物传感器的特定区室亚型，如Panieri等人（2017年）[1]所述，以测试磷酸戊糖途径和谷胱甘肽生物合成对体内心肌细胞和内皮细胞抗氧化能力的贡献。将转基因斑马鱼胚胎进行代谢抑制，随后用H2O2或氧化还原循环剂甲萘醌进行刺激，以分别模拟急性或慢性氧化应激。在受精后48小时对斑马鱼胚胎的心血管组织进行共聚焦延时记录，以跟踪HO和E的区室化变化。在405nm和488nm处进行顺序激发后，每隔2分钟在500-520nm之间收集发射光，总时长为60分钟。将405/488nm比率归一化为添加氧化剂之前获得的初始值，并随时间绘制。数据的分析和解释可在相关文章[1]中找到。
