Hietala J, Uhari M, Tuokko H
Department of Medical Microbiology, University of Oulu, Finland.
Scand J Infect Dis. 1988;20(6):595-9. doi: 10.3109/00365548809035659.
The value of viral antigen detection from nasopharyngeal secretion (NPS) by enzyme immunoassay (EIA) in everyday clinical practice was evaluated in 570 children hospitalized because of infections. NPS-EIA gave a positive result in 32% of all cases. Virus isolation was positive in the NPS in 25%, virus isolation in stool samples in 14%, and virus serology in 28% of the cases. NPS-EIA was superior for detecting adenovirus, respiratory syncytial (RS) and parainfluenza viruses. In a series of 124 patients in whom 3 methods were compared, NPS-EIA was the only positive method in 48% of all findings positive for adenoviruses, in 73% for RSV, and in 58% for parainfluenza viruses. Antigen detection by NPS-EIA markedly increased the diagnostic potential in everyday clinical practice, especially for viral respiratory diseases.
通过酶免疫测定法（EIA）检测570名因感染住院儿童的鼻咽分泌物（NPS）中的病毒抗原，评估其在日常临床实践中的价值。NPS-EIA在所有病例中的阳性率为32%。NPS病毒分离阳性率为25%，粪便样本病毒分离阳性率为14%，病毒血清学阳性率为28%。NPS-EIA在检测腺病毒、呼吸道合胞病毒（RS）和副流感病毒方面更具优势。在124名接受三种方法比较的患者中，NPS-EIA是腺病毒所有阳性结果中48%、RSV阳性结果中73%以及副流感病毒阳性结果中58%的唯一阳性检测方法。NPS-EIA抗原检测显著提高了日常临床实践中的诊断潜力，尤其是对病毒性呼吸道疾病。
