Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences, Little Rock, AR, 72205, USA.
National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, 72132, USA.
J Appl Toxicol. 2017 Nov;37(11):1297-1304. doi: 10.1002/jat.3467. Epub 2017 May 19.
Graphene-based nanomaterials (GBNs) are quickly revolutionizing modern electronics, energy generation and storage, clothing and biomedical devices. Due to GBN's variety of physical and chemical parameters that define their toxicity and their aggregation in suspension, interpreting its toxicology without accurate information on graphene's distribution and behavior in live organisms is challenging. In this work, we present a laser-based optical detection methodology for noninvasive detection and pharmacokinetics analysis of GBNs directly in blood flow in mice using in vivo photoacoustic (PA) flow cytometry (PAFC). PAFC provides unique insight on how chemical modifications of GBNs affect their distribution in blood circulation and how quickly they are eliminated from the flow. Overall, PAFC provided unique data crucial for understanding GBN toxicity through real-time detection of GBNs using their intrinsic light absorption contrast. Copyright © 2017 John Wiley & Sons, Ltd.
基于石墨烯的纳米材料（GBNs）正在迅速改变现代电子、能源的产生和储存、服装和生物医学设备。由于 GBN 的多种物理和化学参数决定了其毒性，并且其在悬浮液中聚集，因此如果没有关于石墨烯在活生物体中分布和行为的准确信息，就很难解释其毒理学。在这项工作中，我们提出了一种基于激光的光学检测方法，用于使用体内光声（PA）流动细胞术（PAFC）直接在血流中对小鼠中的 GBN 进行非侵入性检测和药代动力学分析。PAFC 独特地揭示了 GBN 化学修饰如何影响其在血液循环中的分布，以及它们从血流中清除的速度有多快。总体而言，PAFC 通过使用 GBN 的固有光吸收对比实时检测 GBN，提供了理解 GBN 毒性的关键独特数据。版权所有 © 2017 年 John Wiley & Sons， Ltd.
