基于单步聚合酶链反应的大鼠组织和细胞遗传性别鉴定
Single-step PCR-based genetic sex determination of rat tissues and cells.
作者信息
Dhakal Pramod, Soares Michael J
机构信息
Institute for Reproductive Health and Regenerative Medicine, Department of Pathology & Laboratory Medicine and Department of Pediatrics, University of Kansas Medical Center, Kansas City, KS.
出版信息
Biotechniques. 2017 May 1;62(5):232-233. doi: 10.2144/000114548.
Abstract
The advent of genome editing strategies has expanded the range of animal models available for gene manipulation and renewed research interest in the rat. Gender is a key variable for in vivo gene function analyses. Here, we present a simple PCR-based method to determine genetic sex in the rat.
摘要
基因组编辑策略的出现扩大了可用于基因操作的动物模型范围，并重新激发了对大鼠的研究兴趣。性别是体内基因功能分析的一个关键变量。在此，我们提出一种基于聚合酶链反应（PCR）的简单方法来确定大鼠的遗传性别。
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