Governa M, Valentino M, Visona I, Marchiseppe I, Lo Martire N
Institute of Occupational Medicine, University of Ancona, Torrette di Ancona, Italy.
Cell Biol Toxicol. 1988 Jun;4(2):187-97. doi: 10.1007/BF00119245.
Continuous-filament glass fibers coated with organic agents, candidate asbestos substitutes, were assessed for their ability to elicit from normal human serum complement-derived cleavage products which are able to stimulate the chemotaxis and the respiratory burst of polymorphonuclear leukocytes. Glass fibers generated chemoattracting and respiratory stimulating factors for polymorphonuclears from human serum. The effect was dose related for chemotaxis from the serum fiber concentration of 75 micrograms/ml to 1,250 micrograms/ml. The serum chemoattracting activity, as well the respiratory stimulation, were dramatically impaired when serum had been preliminarily absorbed with antiC5 antiserum. Since the impairment of chemotactic activity occurred also in the presence of EDTA, but not in the presence of EGTA, we assumed an activation of the alternative complement pathway. Glass fibers were studied in comparison to a UICC sample of Canadian chrysotile asbestos, which is able to activate in vitro the alternative complement pathway. Glass fibers exhibited less ability than asbestos fibers to generate complement cleavage products with chemotactic activity for polymorphonuclears; however, they produced an activity about equal to 80% of a chemotactic standard stimulus such as zymosan-activated plasma.
对涂覆有机试剂的连续长丝玻璃纤维（石棉替代候选物）进行了评估，以确定其从正常人血清补体衍生的裂解产物中引发能够刺激多形核白细胞趋化性和呼吸爆发的能力。玻璃纤维可从人血清中产生吸引多形核白细胞的趋化因子和呼吸刺激因子。从血清纤维浓度75微克/毫升至1250微克/毫升，趋化作用呈剂量相关。当血清预先用抗C5抗血清吸收时，血清趋化活性以及呼吸刺激均显著受损。由于趋化活性的损害在存在EDTA时也会发生，但在存在EGTA时不会发生，因此我们假定替代补体途径被激活。与能够在体外激活替代补体途径的加拿大温石棉的国际癌症研究机构（UICC）样本相比，对玻璃纤维进行了研究。玻璃纤维产生具有多形核白细胞趋化活性的补体裂解产物的能力低于石棉纤维；然而，它们产生的活性约等于趋化标准刺激物（如酵母聚糖激活血浆）的80%。
