Cavalu Simona, Prokisch Jόszef, Laslo Vasile, Vicas Simona
Faculty of Medicine and Pharmacy, University of Oradea, P-ta 1 Decembrie 10, 410087, Oradea, Romania.
Institute of Bio- and Environmental Energetics, Debrecen University, Boszormenyi Utca 138, Debrecen 4032, Hungary.
IET Nanobiotechnol. 2017 Jun;11(4):426-432. doi: 10.1049/iet-nbt.2016.0107.
The main goal of this study was to synthesise and characterise different formulations based on alginate and alginate/chitosan microspheres containing nanoselenium (nano-Se) for controlled delivery applications. Nanosize elemental selenium was produced by using probiotic yogurt bacteria () in a fermentation procedure. The structural and morphological characterisation of the microspheres was performed by Fourier transform infrared (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis. FTIR and XRD pattern indicated that was an effective cross-linking of selenium nanoparticles within the polymeric matrix in both cases. The SEM images reveal that selenium nanoparticles are mainly exposed on the surface of alginate, in contrast to porous structure of alginate/chitosan/nano-Se, interconnected in a regular network. This architecture type has a considerable importance in the delivery process, as demonstrated by differential pulse voltammetry. Selenium release from both matrices is pH sensitive. Moreover, chitosan blended with alginate minimise the release of encapsulated selenium, in simulated gastric fluid, and prolong the duration of release in intestinal fluid. The overall effect is the enhancement of total percentage release concomitant with the longer duration of action. The authors' formulation based on alginate/chitosan is a convenient matrix to be used for selenium delivery in duodenum, caecum and colon.
本研究的主要目标是合成并表征基于藻酸盐以及含有纳米硒（nano-Se）的藻酸盐/壳聚糖微球的不同制剂，用于控释应用。通过在发酵过程中使用益生菌酸奶细菌（）制备出纳米尺寸的元素硒。通过傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）和扫描电子显微镜（SEM）分析对微球进行结构和形态表征。FTIR和XRD图谱表明，在两种情况下，纳米硒颗粒在聚合物基质中均实现了有效交联。SEM图像显示，与藻酸盐/壳聚糖/纳米硒的多孔结构相反，纳米硒颗粒主要暴露在藻酸盐表面，藻酸盐/壳聚糖/纳米硒的多孔结构以规则网络相互连接。如差分脉冲伏安法所示，这种结构类型在递送过程中具有相当重要的意义。两种基质中的硒释放均对pH敏感。此外，壳聚糖与藻酸盐混合可使模拟胃液中包封硒的释放最小化，并延长肠液中的释放持续时间。总体效果是提高了总释放百分比并延长了作用持续时间。作者基于藻酸盐/壳聚糖的制剂是一种方便的基质，可用于在十二指肠、盲肠和结肠中递送硒。
