使用临床标本对雅培轮状病毒检测试剂盒进行评估。
Evaluation of Abbott TESTPACK ROTAVIRUS with clinical specimens.
作者信息
Marchlewicz B, Spiewak M, Lampinen J
机构信息
Infectious Disease and Immunology Research, Abbott Laboratories, Abbott Park, Illinois 60064.
出版信息
J Clin Microbiol. 1988 Nov;26(11):2456-8. doi: 10.1128/jcm.26.11.2456-2458.1988.
Abstract
TESTPACK ROTAVIRUS was compared with the Pathfinder Rotavirus assay for the detection of rotaviral antigens in feces from 137 patients. The symptomatology and ages of the patients varied from no symptoms to watery diarrhea and from less than 3 months to greater than 60 years of age. The sensitivity and specificity, before resolution, were 89.2 and 90.0%, respectively. After resolution with a blocking reagent, the results were 100% sensitivity and 98.9% specificity. The performance was similar for all age groups and in both symptomatic and asymptomatic patients. Abbott TESTPACK ROTAVIRUS is a rapid (10-min), easy-to-perform assay for the detection of rotaviral antigens and has the sensitivity and specificity of enzyme-linked immunosorbent assays that require several hours to perform.
摘要
将TESTPACK轮状病毒检测法与Pathfinder轮状病毒检测法进行比较，以检测137例患者粪便中的轮状病毒抗原。患者的症状和年龄各不相同，从无症状到水样腹泻，年龄从不到3个月到60岁以上。在结果确定前，其敏感性和特异性分别为89.2%和90.0%。用封闭试剂处理后，敏感性为100%，特异性为98.9%。在所有年龄组以及有症状和无症状患者中，该检测法的性能相似。雅培TESTPACK轮状病毒检测法是一种快速（10分钟）、易于操作的检测轮状病毒抗原的方法，具有需要数小时才能完成的酶联免疫吸附测定法的敏感性和特异性。
相似文献
1
Evaluation of Abbott TESTPACK ROTAVIRUS with clinical specimens.使用临床标本对雅培轮状病毒检测试剂盒进行评估。
J Clin Microbiol. 1988 Nov;26(11):2456-8. doi: 10.1128/jcm.26.11.2456-2458.1988.
2
Comparison of a new rapid test (TestPack Rotavirus) with standard enzyme immunoassay and electron microscopy for the detection of rotavirus in symptomatic hospitalized children.一种新型快速检测方法（TestPack轮状病毒检测试剂盒）与标准酶免疫测定法及电子显微镜检查法在有症状住院儿童中检测轮状病毒的比较。
J Clin Microbiol. 1989 Apr;27(4):775-7. doi: 10.1128/jcm.27.4.775-777.1989.
3
Ability of TESTPACK ROTAVIRUS enzyme immunoassay to diagnose rotavirus gastroenteritis.TESTPACK轮状病毒酶免疫测定法诊断轮状病毒肠胃炎的能力。
J Clin Microbiol. 1988 Nov;26(11):2459-61. doi: 10.1128/jcm.26.11.2459-2461.1988.
4
Occurrence of nonspecific reactions among stool specimens tested by the Abbott TestPack rotavirus enzyme immunoassay.采用雅培TestPack轮状病毒酶免疫测定法检测的粪便标本中出现非特异性反应。
J Clin Microbiol. 1990 Jun;28(6):1132-4. doi: 10.1128/jcm.28.6.1132-1134.1990.
5
Evaluation of a new enzyme immunoassay (TESTPACK rotavirus) for the detection of rotavirus in fecal specimens.
Diagn Microbiol Infect Dis. 1988 Dec;11(4):201-3. doi: 10.1016/0732-8893(88)90004-1.
6
Comparison of four latex agglutination (LA) and three enzyme-linked immunosorbent assays (ELISA) for the detection of rotavirus in fecal specimens.
Am J Clin Pathol. 1989 Nov;92(5):637-43. doi: 10.1093/ajcp/92.5.637.
7
Comparison of two rapid enzyme immunoassays with standard enzyme immunoassay and latex agglutination for the detection of human rotavirus in stools.
J Diarrhoeal Dis Res. 1994 Jun;12(2):117-20.
8
Comparative evaluation of a commercial enzyme-linked immunoassay and solid-phase immune electron microscopy for rotavirus detection in stool specimens.用于粪便标本中轮状病毒检测的商用酶联免疫吸附测定法和固相免疫电子显微镜法的比较评估
J Clin Microbiol. 1987 Jun;25(6):1137-9. doi: 10.1128/jcm.25.6.1137-1139.1987.
9
Evaluation of a new enzyme immunoassay (TESTPACK ROTAVIRUS) for diagnosis of viral gastroenteritis.
Kansenshogaku Zasshi. 1990 Feb;64(2):174-8. doi: 10.11150/kansenshogakuzasshi1970.64.174.
10
Comparison of electron microscopy with three commercial tests for the detection of rotavirus in animal feces.
Diagn Microbiol Infect Dis. 1987 Mar;6(3):249-54. doi: 10.1016/0732-8893(87)90019-8.
引用本文的文献
1
Advances in the Diagnosis and Treatment of Rotavirus Infections: Narrative Review.轮状病毒感染的诊断与治疗进展：叙述性综述
Int J Mol Sci. 2025 Sep 19;26(18):9175. doi: 10.3390/ijms26189175.
2
The enteritis complex in domestic rabbits: A field study.家兔肠炎综合征：一项实地研究。
Can Vet J. 1993 Feb;34(2):95-102.
3
Genetically related Escherichia coli strains associated with Crohn's disease.与克罗恩病相关的基因相关大肠杆菌菌株。
Gut. 2001 Mar;48(3):320-5. doi: 10.1136/gut.48.3.320.
4
Comparison of a new rapid test (TestPack Rotavirus) with standard enzyme immunoassay and electron microscopy for the detection of rotavirus in symptomatic hospitalized children.一种新型快速检测方法（TestPack轮状病毒检测试剂盒）与标准酶免疫测定法及电子显微镜检查法在有症状住院儿童中检测轮状病毒的比较。
J Clin Microbiol. 1989 Apr;27(4):775-7. doi: 10.1128/jcm.27.4.775-777.1989.
5
Choice of reference assay for the detection of rotavirus in fecal specimens: electron microscopy versus enzyme immunoassay.粪便标本中轮状病毒检测参考检测方法的选择：电子显微镜法与酶免疫分析法的比较
J Clin Microbiol. 1990 Jun;28(6):1280-3. doi: 10.1128/jcm.28.6.1280-1283.1990.
6
Occurrence of nonspecific reactions among stool specimens tested by the Abbott TestPack rotavirus enzyme immunoassay.采用雅培TestPack轮状病毒酶免疫测定法检测的粪便标本中出现非特异性反应。
J Clin Microbiol. 1990 Jun;28(6):1132-4. doi: 10.1128/jcm.28.6.1132-1134.1990.
本文引用的文献
1
Rotavirus carriage, asymptomatic infection, and disease in the first two years of life. I. Virus shedding.轮状病毒携带、无症状感染及生命最初两年的疾病。I. 病毒排出
J Infect Dis. 1984 May;149(5):667-74. doi: 10.1093/infdis/149.5.667.
2
Viral gastroenteritis and laboratory detection of rotavirus.病毒性肠胃炎与轮状病毒的实验室检测
Am J Med Technol. 1983 Sep;49(9):665-8.
3
Evaluation of a new latex agglutination test for detecting human rotavirus in faeces.一种用于检测粪便中人类轮状病毒的新型乳胶凝集试验的评估。
J Infect. 1983 Sep;7(2):130-3. doi: 10.1016/s0163-4453(83)90527-3.
4
Rotavirus as a cause of severe gastroenteritis in adults.轮状病毒作为成人严重肠胃炎的病因
J Clin Microbiol. 1983 Sep;18(3):663-7. doi: 10.1128/jcm.18.3.663-667.1983.
5
Spread of rotavirus within families: a community based study.轮状病毒在家庭中的传播：一项基于社区的研究。
Br Med J (Clin Res Ed). 1983 Aug 27;287(6392):575-7. doi: 10.1136/bmj.287.6392.575.
6
Rapid detection of rotavirus in stool by latex agglutination: comparison with radioimmunoassay and electron microscopy and clinical evaluation of the test.通过乳胶凝集法快速检测粪便中的轮状病毒：与放射免疫测定法和电子显微镜检查的比较及该检测方法的临床评估
J Med Virol. 1983;11(2):91-7. doi: 10.1002/jmv.1890110202.
7
Comparison of an enzyme immunoassay with electron microscopic procedures for detecting rotavirus.酶免疫测定法与电子显微镜检测程序在轮状病毒检测中的比较。
J Clin Microbiol. 1982 May;15(5):938-44. doi: 10.1128/jcm.15.5.938-944.1982.
8
Rotavirus infection in a geriatric population.
Arch Intern Med. 1982 Feb;142(2):313-6.
9
An epidemic of rotavirus-associated gastroenteritis in a nursing home for the elderly.
Scand J Infect Dis. 1980;12(3):161-4. doi: 10.3109/inf.1980.12.issue-3.01.
10
Confirmatory testing of Rotazyme results in neonates.
J Pediatr. 1985 Aug;107(2):289-92. doi: 10.1016/s0022-3476(85)80153-0.
Zotero 插件