TESTPACK轮状病毒酶免疫测定法诊断轮状病毒肠胃炎的能力。
Ability of TESTPACK ROTAVIRUS enzyme immunoassay to diagnose rotavirus gastroenteritis.
作者信息
Chernesky M, Castriciano S, Mahony J, Spiewak M, Schaefer L
机构信息
McMaster University Regional Virology Laboratory, St. Joseph's Hospital, Hamilton, Ontario, Canada.
出版信息
J Clin Microbiol. 1988 Nov;26(11):2459-61. doi: 10.1128/jcm.26.11.2459-2461.1988.
Abstract
TESTPACK ROTAVIRUS, a simple 10-min enzyme immunoassay, was compared with electron microscopy and Pathfinder enzyme immunoassay on feces from 172 patients of various ages with gastroenteritis. The percent sensitivities and specificities before blocking with antiserum were as follows: TESTPACK, 100% sensitivity and 99% specificity; Pathfinder, 95% sensitivity and 98% specificity. After blocking, the sensitivity and specificity, respectively, were 100% and 100% for TESTPACK and 95% and 99% for Pathfinder. TESTPACK ROTAVIRUS was more sensitive, but not significantly, than Pathfinder (P greater than 0.1) and the direct electron microscopy technique (P greater than 0.1).
摘要
TESTPACK轮状病毒检测，一种简单的10分钟酶免疫测定法，与电子显微镜检查法以及Pathfinder酶免疫测定法对172名不同年龄的肠胃炎患者的粪便进行了比较。在用抗血清封闭之前，其灵敏度和特异性百分比分别如下：TESTPACK，灵敏度100%，特异性99%；Pathfinder，灵敏度95%，特异性98%。封闭后，TESTPACK的灵敏度和特异性分别为100%和100%，Pathfinder的灵敏度和特异性分别为95%和99%。TESTPACK轮状病毒检测比Pathfinder（P>0.1）和直接电子显微镜技术（P>0.1）更敏感，但差异不显著。
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