Chen Tingtao, Shi Yan, Wang Xiaolei, Wang Xin, Meng Fanjing, Yang Shaoguo, Yang Jian, Xin Hongbo
Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, P.R. China.
Department of Conservative Dentistry and Endodontics, Stomatological Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
Mol Med Rep. 2017 Jul;16(1):127-132. doi: 10.3892/mmr.2017.6593. Epub 2017 May 17.
Recurrence of oral diseases caused by antibiotics has brought about an urgent requirement to explore the oral microbial diversity in the human oral cavity. In the present study, the high‑throughput sequencing method was adopted to compare the microbial diversity of healthy people and oral patients and sequence analysis was performed by UPARSE software package. The Venn results indicated that a mean of 315 operational taxonomic units (OTUs) was obtained, and 73, 64, 53, 19 and 18 common OTUs belonging to Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria and Fusobacteria, respectively, were identified in healthy people. Moreover, the reduction of Firmicutes and the increase of Proteobacteria in the children group, and the increase of Firmicutes and the reduction of Proteobacteria in the youth and adult groups, indicated that the age bracket and oral disease had largely influenced the tooth development and microbial development in the oral cavity. In addition, the traditional 'pathogenic bacteria' of Firmicutes, Proteobacteria and Bacteroidetes (accounted for >95% of the total sequencing number in each group) indicated that the 'harmful' bacteria may exert beneficial effects on oral health. Therefore, the data will provide certain clues for curing some oral diseases by the strategy of adjusting the disturbed microbial compositions in oral disease to healthy level.
抗生素引起的口腔疾病复发促使人们迫切需要探索人类口腔中的微生物多样性。在本研究中，采用高通量测序方法比较健康人和口腔疾病患者的微生物多样性，并通过UPARSE软件包进行序列分析。Venn结果表明，平均获得了315个可操作分类单元（OTU），在健康人群中分别鉴定出属于厚壁菌门、拟杆菌门、变形菌门、放线菌门和梭杆菌门的73、64、53、19和18个常见OTU。此外，儿童组中厚壁菌门减少而变形菌门增加，青年组和成人组中厚壁菌门增加而变形菌门减少，这表明年龄范围和口腔疾病在很大程度上影响了口腔中的牙齿发育和微生物发育。此外，厚壁菌门、变形菌门和拟杆菌门这些传统的“病原菌”（在每组中占总测序数的>95%）表明，这些“有害”细菌可能对口腔健康产生有益影响。因此，这些数据将为通过将口腔疾病中紊乱的微生物组成调整到健康水平的策略来治疗某些口腔疾病提供一定线索。
