利用CRISPR/Cas9系统在[具体物种]中生成新发现的抗性基因敲除体
Generation of Newly Discovered Resistance Gene Knockout in Using the CRISPR/Cas9 System.
作者信息
Sun Lichang, He Tao, Zhang Lili, Pang Maoda, Zhang Qiaoyan, Zhou Yan, Bao Hongduo, Wang Ran
机构信息
Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R. China.
Zhejiang Province Key Laboratory for Food Safety, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, P.R. China.
出版信息
J Microbiol Biotechnol. 2017 Jul 28;27(7):1276-1280. doi: 10.4014/jmb.1611.11021.
The gene is a new "superbug" gene discoverd in China in 2016 that makes bacteria highly resistant to the last-resort class of antibiotics. The gene raised serious concern about its possible global dissemination and spread. Here, we report a potential anti-resistant strategy using the CRISPR/Cas9-mediated approach that can efficiently induce gene knockout in . Our findings suggested that using the CRISPR/Cas9 system to knock out the resistance gene might be a potential anti-resistant strategy. Bovine myeloid antimicrobial peptide-27 could help deliver plasmid pCas::mcr targeting specific DNA sequences of the gene into microbial populations.
该基因是2016年在中国发现的一种新的“超级细菌”基因，它使细菌对最后一道防线类抗生素具有高度抗性。该基因引发了对其可能在全球传播和扩散的严重担忧。在此，我们报告一种使用CRISPR/Cas9介导方法的潜在抗耐药策略，该方法可有效诱导基因敲除。我们的研究结果表明，使用CRISPR/Cas9系统敲除耐药基因可能是一种潜在的抗耐药策略。牛髓样抗菌肽-27可帮助将靶向该基因特定DNA序列的质粒pCas::mcr递送至微生物群体中。
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