Michael Smith Laboratories, The University of British Columbia, 2185 East Mall, V6T 1Z4, Vancouver, BC, Canada.
Analyst. 2017 Jun 12;142(12):2199-2210. doi: 10.1039/c7an00349h.
Blood banking is an essential aspect of modern medical care. When red blood cells (RBCs) are stored, they become damaged by various chemical processes, such as accumulation of their own waste products and oxidative injury, among others. These processes lead to the development of the RBC storage lesion, a complex condition where the severity is reflected through the morphology of the stored cells. It was hypothesized that Raman spectroscopy could be used to monitor certain structural and compositional changes associated with such ageing effects and that a relationship between these features and traditional morphology (as measured using a morphology index) could be observed. The hypothesis was tested by measuring spectral features associated with hemoglobin oxygenation from dry-fixed smears and liquid RBCs for twenty-nine different donors (combined), and comparing the trends with morphological scoring from seven of these donors. After appropriately fitting the two data sets to either power or linear curves, the oxygenation state was shown to change in a manner that was donor-dependent and that closely tracked morphological changes. This study suggests Raman analysis has promise for providing a rapid and objective measure of the cell quality of stored RBCs through measurements of hemoglobin oxygenation that is comparable to traditional morphological assessment.
血液银行是现代医疗保健的重要组成部分。当红细胞（RBC）被储存时，它们会受到各种化学过程的损害，例如自身废物的积累和氧化损伤等。这些过程导致 RBC 储存损伤的发展，这是一种严重程度通过储存细胞的形态反映出来的复杂情况。据推测，拉曼光谱可以用于监测与这种老化效应相关的某些结构和组成变化，并且可以观察到这些特征与传统形态（使用形态指数测量）之间的关系。通过测量来自 29 位不同供体（组合）的干固定涂片和液体 RBC 与血红蛋白氧合相关的光谱特征，并将趋势与其中 7 位供体的形态评分进行比较，对该假设进行了测试。对两个数据集进行适当拟合后，氧合状态显示出与供体依赖性相关的变化，并且与形态变化密切相关。这项研究表明，通过对血红蛋白氧合的测量，拉曼分析有望通过测量血红蛋白氧合来提供一种快速、客观的方法，来评估储存 RBC 的细胞质量，这种方法与传统的形态评估相当。
