Mauricio María D, Serna Eva, Fernández-Murga María Leonor, Portero Jesica, Aldasoro Martín, Valles Soraya L, Sanz Yolanda, Vila José M
Departamento de Fisiología, Universitat de Valencia, Valencia, Spain.
Fundación de Investigación del Hospital Clínico Universitario de Valencia/INCLIVA, Valencia, Spain.
Int J Med Sci. 2017 Apr 8;14(5):444-451. doi: 10.7150/ijms.18354. eCollection 2017.
CECT 7765 improves metabolic and immunological altered functions in high fat fed mice, however little is known about the effects of potential probiotics on vascular reactivity. The aim of the present study was to investigate the effects of a potential probiotic strain, CECT 7765, on vascular response in obese mice. Aorta samples were obtained from mice, which were divided into three groups: a control group, receiving a standard diet; an obese group, receiving a high-fat diet; and an obese group receiving high-fat diet and a daily dose of B. pseudocatenulatum CECT 7765 by oral gavage. Aortic rings were suspended in organ baths for isometric recording of tension. mRNA expression of eNOS was evaluated by real-time polymerase chain reaction. Contractions induced by KCl, noradrenaline and thromboxane analogue were 33%, 30% and 45% lower respectively in aortic rings from obese mice. Bifidobacteria administration reversed this effect. eNOS inhibition increased the response to noradrenaline in the three groups with a significant lower magnitude in aortic rings from obese mice receiving bifidobacteria supplement. Acetylcholine caused a greater vasodilation in aorta from obese group (46±3% for control and 69±4% for obese group; p<0.05) and bifidobacteria reversed it (57±5%). Response to sodium nitroprusside was displaced 2.9 times to the left in a parallel manner in obese group. Relaxation to sodium nitroprusside remained unchanged in the bifidobacteria fed group. There was about five-fold decreased mRNA expression of eNOS in aortic segments from the group receiving bifidobacteria. pseudocatenulatum CECT 7765 restores the obesity-induced altered vascular function mainly by reducing nitric oxide release.
CECT 7765可改善高脂喂养小鼠的代谢和免疫功能改变，但关于潜在益生菌对血管反应性的影响知之甚少。本研究的目的是调查潜在益生菌菌株CECT 7765对肥胖小鼠血管反应的影响。从小鼠获取主动脉样本，将其分为三组：对照组，给予标准饮食；肥胖组，给予高脂饮食；肥胖组给予高脂饮食并通过口服灌胃每日一剂假链状双歧杆菌CECT 7765。将主动脉环悬挂在器官浴槽中进行张力的等长记录。通过实时聚合酶链反应评估eNOS的mRNA表达。肥胖小鼠主动脉环中由氯化钾、去甲肾上腺素和血栓素类似物诱导的收缩分别降低了33%、30%和45%。给予双歧杆菌可逆转这种作用。在三组中，eNOS抑制均增加了对去甲肾上腺素的反应，在接受双歧杆菌补充的肥胖小鼠主动脉环中幅度显著降低。乙酰胆碱在肥胖组主动脉中引起更大的血管舒张（对照组为46±3%，肥胖组为69±4%；p<0.05），双歧杆菌使其逆转（57±5%）。肥胖组中对硝普钠的反应以平行方式向左移动了2.9倍。在给予双歧杆菌的组中，对硝普钠的舒张反应保持不变。在接受双歧杆菌的组的主动脉段中，eNOS的mRNA表达下降了约五倍。假链状双歧杆菌CECT 7765主要通过减少一氧化氮释放来恢复肥胖诱导的血管功能改变。
