Ahmed Abd-Almohaimen, Hamzah Haider, Maaroof Mohammed
Department of Biology, College of Education for Pure Sciences, Tikrit University , Tikrit , Iraq.
Department of Biology, College of Science, University of Sulaimani , Sulaimani, Kurdistan Region , Iraq.
Turk J Biol. 2018 Feb 15;42(1):54-62. doi: 10.3906/biy-1710-2. eCollection 2018.
In recent years much attention has been paid to the biosynthesis of silver nanoparticles (AgNPs) and their important medical applications. The current study employs Fusarium oxysporum for the formation of silver nanoparticles and examines the antimicrobial activity of the particles against some multidrug-resistant (MDR) microbes. Silver nitrate was transformed into silver oxide, forming well-dispersed nanoparticles, by the action of F. oxysporum metabolically. The size of the nanoparticles ranged from 21.3 to 37.3 nm, and UV-spectroscopy showed a peak at 408-411 nm. Moreover, SEM, TEM, and AFM results revealed spherical and oval shapes and showed no sign of aggregation. Furthermore, the FT-IR histogram detected amide I and amide II, which are responsible for the stability of AgNPs in the aqueous solution. The AgNPs halted the growth of MDR bacteria, including some members of Enterobacteriaceae and Staphylococcus species at a concentration of 50% (v/v). The AgNPs also have the ability to inhibit pathogenic yeasts Candida albicans and Candida krusei. The AgNPs displayed antigrowth activity against MDR microbes, suggesting that they might be potential alternatives to antibiotics. However, additional studies may be necessary to substantiate the fact that the benefits of using nanoparticles outweigh the potential risks.
近年来，银纳米颗粒（AgNPs）的生物合成及其重要的医学应用受到了广泛关注。当前的研究利用尖孢镰刀菌来制备银纳米颗粒，并检测了这些颗粒对一些多重耐药（MDR）微生物的抗菌活性。通过尖孢镰刀菌的代谢作用，硝酸银被转化为氧化银，形成了分散良好的纳米颗粒。纳米颗粒的尺寸范围为21.3至37.3纳米，紫外光谱显示在408 - 411纳米处有一个峰值。此外，扫描电子显微镜（SEM）、透射电子显微镜（TEM）和原子力显微镜（AFM）的结果显示颗粒呈球形和椭圆形，且没有聚集的迹象。此外，傅里叶变换红外光谱（FT - IR）直方图检测到了酰胺I和酰胺II，它们负责AgNPs在水溶液中的稳定性。AgNPs在浓度为50%（v/v）时能抑制包括肠杆菌科的一些成员和葡萄球菌属在内的多重耐药细菌的生长。AgNPs还具有抑制致病性酵母白色念珠菌和克鲁斯念珠菌的能力。AgNPs对多重耐药微生物显示出抗生长活性，这表明它们可能是抗生素的潜在替代品。然而，可能需要进一步的研究来证实使用纳米颗粒的益处大于潜在风险这一事实。
