胚胎干细胞分化中的神经酰胺和1-磷酸鞘氨醇信号传导
Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation.
作者信息
Wang Guanghu, Spassieva Stefka D, Bieberich Erhard
机构信息
Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA.
Department of Molecular and Cellular Medicine, Texas A&M Medical Health Sciences Center, Bryan, TX, USA.
出版信息
Methods Mol Biol. 2018;1697:153-171. doi: 10.1007/7651_2017_43.
Abstract
Bioactive sphingolipids are important regulators for stem cell survival and differentiation. Most recently, we have coined the term "morphogenetic lipids" for sphingolipids that regulate stem cells during embryonic and postnatal development. The sphingolipid ceramide and its derivative, sphingosine-1-phosphate (S1P), can act synergistically as well as antagonistically on embryonic stem (ES) cell differentiation. We show here simple as well as state-of-the-art methods to analyze sphingolipids in differentiating ES cells and discuss new protocols to use ceramide and S1P analogs for the guided differentiation of mouse ES cells toward neuronal and glial lineage.
摘要
生物活性鞘脂是干细胞存活和分化的重要调节因子。最近，我们创造了“形态发生脂质”一词来指代在胚胎发育和出生后发育过程中调节干细胞的鞘脂。鞘脂神经酰胺及其衍生物鞘氨醇-1-磷酸（S1P），在胚胎干细胞（ES细胞）分化过程中既能协同作用，也能产生拮抗作用。我们在此展示了分析分化中的ES细胞中鞘脂的简单方法以及先进方法，并讨论了使用神经酰胺和S1P类似物引导小鼠ES细胞向神经元和神经胶质谱系分化的新方案。
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