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特殊的C/D盒小核仁核糖核蛋白（snoRNPs）作为反义向导靶向RNA碱基乙酰化。

Specialized box C/D snoRNPs act as antisense guides to target RNA base acetylation.

作者信息

Sharma Sunny, Yang Jun, van Nues Rob, Watzinger Peter, Kötter Peter, Lafontaine Denis L J, Granneman Sander, Entian Karl-Dieter

机构信息

Institute of Molecular Biosciences, Goethe University, Frankfurt am Main, Germany.

RNA Molecular Biology and CMMI, F.R.S./FNRS and Université Libre de Bruxelles Rue Profs Jeener & Brachet, Charleroi-Gosselies, Belgium.

出版信息

PLoS Genet. 2017 May 24;13(5):e1006804. doi: 10.1371/journal.pgen.1006804. eCollection 2017 May.

DOI:10.1371/journal.pgen.1006804
PMID:28542199
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5464676/
Abstract

Box C/D snoRNAs are known to guide site-specific ribose methylation of ribosomal RNA. Here, we demonstrate a novel and unexpected role for box C/D snoRNAs in guiding 18S rRNA acetylation in yeast. Our results demonstrate, for the first time, that the acetylation of two cytosine residues in 18S rRNA catalyzed by Kre33 is guided by two orphan box C/D snoRNAs-snR4 and snR45 -not known to be involved in methylation in yeast. We identified Kre33 binding sites on these snoRNAs as well as on the 18S rRNA, and demonstrate that both snR4 and snR45 establish extended bipartite complementarity around the cytosines targeted for acetylation, similar to pseudouridylation pocket formation by the H/ACA snoRNPs. We show that base pairing between these snoRNAs and 18S rRNA requires the putative helicase activity of Kre33, which is also needed to aid early pre-rRNA processing. Compared to yeast, the number of orphan box C/D snoRNAs in higher eukaryotes is much larger and we hypothesize that several of these may be involved in base-modifications.

摘要

C/D 盒小核仁RNA（snoRNAs）已知可引导核糖体RNA的位点特异性核糖甲基化。在此，我们证明了C/D盒snoRNAs在引导酵母中18S核糖体RNA（rRNA）乙酰化方面具有新的意外作用。我们的结果首次证明，Kre33催化的18S rRNA中两个胞嘧啶残基的乙酰化由两个孤儿C/D盒snoRNAs——snR4和snR45引导，而这两个snoRNAs在酵母中并不参与甲基化。我们确定了这些snoRNAs以及18S rRNA上的Kre33结合位点，并证明snR4和snR45在靶向乙酰化的胞嘧啶周围都形成了延伸的二分互补性，类似于H/ACA snoRNP形成假尿嘧啶化口袋。我们表明，这些snoRNAs与18S rRNA之间的碱基配对需要Kre33的假定解旋酶活性，这对于帮助早期前体rRNA加工也是必需的。与酵母相比，高等真核生物中孤儿C/D盒snoRNAs的数量要多得多，我们推测其中一些可能参与碱基修饰。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0907/5464676/39ee8ba81f26/pgen.1006804.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0907/5464676/868cb9722e77/pgen.1006804.g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0907/5464676/0b37d891a5b7/pgen.1006804.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0907/5464676/85ebcc181b8b/pgen.1006804.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0907/5464676/97b76500ad2f/pgen.1006804.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0907/5464676/ab4e1a58f7b9/pgen.1006804.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0907/5464676/91425620106a/pgen.1006804.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0907/5464676/39ee8ba81f26/pgen.1006804.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0907/5464676/868cb9722e77/pgen.1006804.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0907/5464676/89d2f702875f/pgen.1006804.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0907/5464676/0b37d891a5b7/pgen.1006804.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0907/5464676/85ebcc181b8b/pgen.1006804.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0907/5464676/97b76500ad2f/pgen.1006804.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0907/5464676/ab4e1a58f7b9/pgen.1006804.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0907/5464676/91425620106a/pgen.1006804.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0907/5464676/39ee8ba81f26/pgen.1006804.g008.jpg

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