Oncology Department of Shanghai Jiao Tong University Affiliated Sixth People's Hospital of Shanghai, Shanghai 200233, China.
Gynecology and Obstetrics Department of the First Affiliated Hospital of Jinan University, Guangzhou 510630, China.
Acta Pharmacol Sin. 2017 Oct;38(10):1394-1400. doi: 10.1038/aps.2017.60. Epub 2017 May 29.
Resveratrol has long been known as an antioxidant and a chemopreventive agent. Similar to resveratrol, pterostilbene (PT) is also a phenolic compound extracted from the Vitis species. However, there are few studies on the antitumor effect of PT. Thus, we investigated the effects of PT on the endometrial cancer (EC) cells in vitro and the related molecular mechanisms. Treatment of EC cell lines HTB-111 and Ishikawa with PT (25-100 μmol/L) dose-dependently suppressed the cell viability and induced apoptosis. Using miR microarrays, we examined the miR expression profile in Ishikawa cells with or without PT, and revealed that miR-663b was the most decreased in PT-treated Ishikawa cells. Furthermore, we predicted and verified that the pro-apoptosis factor BCL2L14 is the direct target of miR-663b. Over-expression of miR-663b and knock-down of BCL2L14 counteracted the suppressing effects of PT on HTB-111 and Ishikawa cells. In addition, we evaluated the miR-663b levels in EC tissues of 51 patients using an in situ hybridization technique. With the median of the score of miR-663b as a cut-off value, these EC patients were divided into two groups, and the patients with high miR-663b expression had significantly poor prognosis.
白藜芦醇长期以来一直被认为是一种抗氧化剂和化学预防剂。与白藜芦醇类似，紫檀芪（PT）也是从葡萄属植物中提取的一种酚类化合物。然而，关于 PT 的抗肿瘤作用的研究很少。因此，我们研究了 PT 对体外子宫内膜癌（EC）细胞的影响及其相关的分子机制。用 PT（25-100μmol/L）处理 EC 细胞系 HTB-111 和 Ishikawa，可剂量依赖性地抑制细胞活力并诱导细胞凋亡。使用 miR 微阵列，我们检测了用或不用 PT 处理的 Ishikawa 细胞中的 miR 表达谱，并发现 miR-663b 在 PT 处理的 Ishikawa 细胞中表达下降最明显。此外，我们预测并验证了促凋亡因子 BCL2L14 是 miR-663b 的直接靶标。miR-663b 的过表达和 BCL2L14 的敲低可拮抗 PT 对 HTB-111 和 Ishikawa 细胞的抑制作用。此外，我们使用原位杂交技术评估了 51 例 EC 组织中的 miR-663b 水平。以 miR-663b 评分的中位数为截止值，将这些 EC 患者分为两组，miR-663b 高表达的患者预后明显较差。
